Cloning and expression of a cDNA encoding a catalytically active fragment of calf thymus DNA polymerase alpha.

Source:http://linkedlifedata.com/resource/pubmed/id/3096317

Biochem. Biophys. Res. Commun. 1986 Oct 15 140 1 21-7

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Authors

Ferrari E, Brown N, Khan N, Hübscher U, Barnes M, Foster K, McMaster G, Eliassen K, Lüthi-Steinmann K

Abstract

A calf thymus cDNA expression library was constructed in the EcoRI site of lambda gt11 and probed with an antibody raised against calf thymus DNA polymerase alpha. Three classes of antibody-reactive clones were isolated. The largest class carried a 1.9 kilobase calf cDNA insert and expressed a 165-175 kilodalton beta-galactosidase:calf fusion protein which displayed DNA polymerase activity. The characteristic responses of the polymerase activity to alpha-specific inhibitors and antibodies identified the 1.9 kilobase cDNA as a sequence specifically derived from the structural gene encoding the pol alpha catalytic core.

PMID
3096317

Publication types

Research Support, U.S. Gov't, P.H.S.; Research Support, Non-U.S. Gov't