Source:http://linkedlifedata.com/resource/pubmed/id/3096317
Biochem. Biophys. Res. Commun. 1986 Oct 15 140 1 21-7
General Info
Abstract
A calf thymus cDNA expression library was constructed in the EcoRI site of lambda gt11 and probed with an antibody raised against calf thymus DNA polymerase alpha. Three classes of antibody-reactive clones were isolated. The largest class carried a 1.9 kilobase calf cDNA insert and expressed a 165-175 kilodalton beta-galactosidase:calf fusion protein which displayed DNA polymerase activity. The characteristic responses of the polymerase activity to alpha-specific inhibitors and antibodies identified the 1.9 kilobase cDNA as a sequence specifically derived from the structural gene encoding the pol alpha catalytic core.
PMID
3096317
Publication types
Research Support, U.S. Gov't, P.H.S.; Research Support, Non-U.S. Gov't