pubmed-article:3094959 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:3094959 | lifeskim:mentions | umls-concept:C1135918 | lld:lifeskim |
pubmed-article:3094959 | lifeskim:mentions | umls-concept:C0178719 | lld:lifeskim |
pubmed-article:3094959 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
pubmed-article:3094959 | lifeskim:mentions | umls-concept:C0025973 | lld:lifeskim |
pubmed-article:3094959 | lifeskim:mentions | umls-concept:C1710425 | lld:lifeskim |
pubmed-article:3094959 | lifeskim:mentions | umls-concept:C2003851 | lld:lifeskim |
pubmed-article:3094959 | pubmed:issue | 4 | lld:pubmed |
pubmed-article:3094959 | pubmed:dateCreated | 1986-12-9 | lld:pubmed |
pubmed-article:3094959 | pubmed:abstractText | Novel, double-barrelled Ca2+-selective microelectrodes with tip diameters of approximately 0.1 micron were constructed by using Simon's neutral Ca2+ ligand (ETH 1001). Concentric micropipettes were utilized for the first time for Ca2+-selective microelectrodes in which the Ca2+ ligand was incorporated into a protruding inner pipette, surrounded by an outer reference electrode. In addition, they were made from high resistance aluminosilicate glass tubing (Corning Code 1724). These Ca2+-selective electrodes had linear responses from pCa 3 to pCa 7 in the presence of constant [K+]. They provided on-line observation of changes in intracellular [Ca2+] and in the resting membrane potential in single smooth muscle cells isolated from toad stomach. The mean concentration of intracellular Ca2+ in resting cells was 163.6 +/- 20 nM (+/- SEM, n = 16). Doubling the intracellular Ca2+ level by exposure of cells to elevated [K+] was sufficient to cause shortening. | lld:pubmed |
pubmed-article:3094959 | pubmed:language | eng | lld:pubmed |
pubmed-article:3094959 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:3094959 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:3094959 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:3094959 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:3094959 | pubmed:month | Aug | lld:pubmed |
pubmed-article:3094959 | pubmed:issn | 0143-4160 | lld:pubmed |
pubmed-article:3094959 | pubmed:author | pubmed-author:YamaguchiHH | lld:pubmed |
pubmed-article:3094959 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:3094959 | pubmed:volume | 7 | lld:pubmed |
pubmed-article:3094959 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:3094959 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:3094959 | pubmed:pagination | 203-19 | lld:pubmed |
pubmed-article:3094959 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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pubmed-article:3094959 | pubmed:year | 1986 | lld:pubmed |
pubmed-article:3094959 | pubmed:articleTitle | Recording of intracellular Ca2+ from smooth muscle cells by sub-micron tip, double-barrelled CA2+-selective microelectrodes. | lld:pubmed |
pubmed-article:3094959 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:3094959 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
pubmed-article:3094959 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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