| pubmed-article:3063604 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3063604 | lifeskim:mentions | umls-concept:C0036025 | lld:lifeskim |
| pubmed-article:3063604 | lifeskim:mentions | umls-concept:C0015576 | lld:lifeskim |
| pubmed-article:3063604 | lifeskim:mentions | umls-concept:C0079866 | lld:lifeskim |
| pubmed-article:3063604 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:3063604 | lifeskim:mentions | umls-concept:C1707689 | lld:lifeskim |
| pubmed-article:3063604 | lifeskim:mentions | umls-concept:C0442335 | lld:lifeskim |
| pubmed-article:3063604 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:3063604 | pubmed:dateCreated | 1989-3-2 | lld:pubmed |
| pubmed-article:3063604 | pubmed:abstractText | A simple assay called the sectoring shuffle was developed to monitor the mutational state of essential genes cloned into yeast centromeric plasmids. The essence of this assay is the creation of a conditional phenotype, colony color sectoring, for an essential gene in the absence of conditional thermosensitive or cold-sensitive alleles of that gene. This allows the quick determination of the mutational state of a cloned essential gene by observing its effect on the sectoring phenotype of the tester strain. During the course of this work we developed a family of 20 Escherichia coli-yeast shuttle vectors, pUN plasmids, containing ARS1 CEN4 and a variety of selectable markers as well as the SUP11 gene which can act as a color marker in the proper background. These vectors are compact and have been very useful for the sectoring-shuffle assay and for gene analysis in general. This paper describes these vectors, the sectoring shuffle and several applications of sectoring phenotypes. | lld:pubmed |
| pubmed-article:3063604 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3063604 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3063604 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3063604 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3063604 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3063604 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:3063604 | pubmed:issn | 0378-1119 | lld:pubmed |
| pubmed-article:3063604 | pubmed:author | pubmed-author:DavisR WRW | lld:pubmed |
| pubmed-article:3063604 | pubmed:author | pubmed-author:ElledgeS JSJ | lld:pubmed |
| pubmed-article:3063604 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3063604 | pubmed:day | 30 | lld:pubmed |
| pubmed-article:3063604 | pubmed:volume | 70 | lld:pubmed |
| pubmed-article:3063604 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3063604 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3063604 | pubmed:pagination | 303-12 | lld:pubmed |
| pubmed-article:3063604 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:3063604 | pubmed:meshHeading | pubmed-meshheading:3063604-... | lld:pubmed |
| pubmed-article:3063604 | pubmed:meshHeading | pubmed-meshheading:3063604-... | lld:pubmed |
| pubmed-article:3063604 | pubmed:meshHeading | pubmed-meshheading:3063604-... | lld:pubmed |
| pubmed-article:3063604 | pubmed:meshHeading | pubmed-meshheading:3063604-... | lld:pubmed |
| pubmed-article:3063604 | pubmed:meshHeading | pubmed-meshheading:3063604-... | lld:pubmed |
| pubmed-article:3063604 | pubmed:meshHeading | pubmed-meshheading:3063604-... | lld:pubmed |
| pubmed-article:3063604 | pubmed:meshHeading | pubmed-meshheading:3063604-... | lld:pubmed |
| pubmed-article:3063604 | pubmed:meshHeading | pubmed-meshheading:3063604-... | lld:pubmed |
| pubmed-article:3063604 | pubmed:meshHeading | pubmed-meshheading:3063604-... | lld:pubmed |
| pubmed-article:3063604 | pubmed:meshHeading | pubmed-meshheading:3063604-... | lld:pubmed |
| pubmed-article:3063604 | pubmed:meshHeading | pubmed-meshheading:3063604-... | lld:pubmed |
| pubmed-article:3063604 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:3063604 | pubmed:articleTitle | A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae. | lld:pubmed |
| pubmed-article:3063604 | pubmed:affiliation | Department of Biochemistry, Stanford University School of Medicine, CA 94305. | lld:pubmed |
| pubmed-article:3063604 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3063604 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:3063604 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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