Linked Life Data

3059715

Source:http://linkedlifedata.com/resource/pubmed/id/3059715

Statements in which the resource exists.
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pubmed-article:3059715pubmed:abstractTextSterol uptake control mutants (upc-) have been isolated via ethylmethanesulfonate mutagenesis from wild-type Saccharomyces cerevisiae. These mutants are heme and sterol competent but possess the ability to accumulate exogenous sterol(s) under aerobic conditions. Previous studies demonstrate sterol uptake only in a hem-, erg- background; however, the Upc- strains described here are Hem+ and do not require exogenous sterol for growth. We were unable to obtain viable hem+, erg-, upc+ recombinants; such combinations appear to be lethal. Isolates of Upc mutants demonstrated different levels of sterol uptake, and sterol analysis revealed a broad phenotypic range with regard to amounts and accumulation of ergosterol and non-ergosterol sterols. Assays of acyl CoA: ergosterol acyltransferase and sterol ester hydrolase showed no apparent difference in activity between Upc mutants and the wild type.lld:pubmed
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pubmed-article:3059715pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:3059715pubmed:articleTitlePleiotropic mutations in Saccharomyces cerevisiae affecting sterol uptake and metabolism.lld:pubmed
pubmed-article:3059715pubmed:affiliationDepartment of Microbiology, North Carolina State University, Raleigh 27695.lld:pubmed
pubmed-article:3059715pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:3059715pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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