pubmed-article:305893 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:305893 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
pubmed-article:305893 | lifeskim:mentions | umls-concept:C0024264 | lld:lifeskim |
pubmed-article:305893 | lifeskim:mentions | umls-concept:C1521761 | lld:lifeskim |
pubmed-article:305893 | lifeskim:mentions | umls-concept:C1947989 | lld:lifeskim |
pubmed-article:305893 | lifeskim:mentions | umls-concept:C1999177 | lld:lifeskim |
pubmed-article:305893 | lifeskim:mentions | umls-concept:C0728938 | lld:lifeskim |
pubmed-article:305893 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
pubmed-article:305893 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
pubmed-article:305893 | pubmed:issue | 3 | lld:pubmed |
pubmed-article:305893 | pubmed:dateCreated | 1978-6-12 | lld:pubmed |
pubmed-article:305893 | pubmed:abstractText | The cloning potential of PHA-treated T cells is significantly enhanced when lymphocyte culture fluid (LCF) from mitogen-treated lymphocytes is added to the soft agar culture system. The mitogens seem to stimulate the release of a lymphocyte colony enhancing factor (LCEF) into the culture medium. A study of the physico-chemical properties of the LCEF revealed that it is a nondialyzable, heat-labile molecule which migrates in the haptoglobin (2--2) post-transferrin region in acrylamide electrophoresis. It is stable to RNase and DNase but labile to papain and trypsin. The LCEF was partially purified from the crude LCF using a sequence of techniques--ammonium sulphate precipitation, DEAE-cellulose and Bio-Gel P-150 chromatography and disc electrophoresis. The mol. wt of the purified LCEF, determined from gel filtration chromatography, was 90,000--110,000. | lld:pubmed |
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pubmed-article:305893 | pubmed:language | eng | lld:pubmed |
pubmed-article:305893 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:305893 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:305893 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:305893 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:305893 | pubmed:month | Mar | lld:pubmed |
pubmed-article:305893 | pubmed:issn | 0019-2805 | lld:pubmed |
pubmed-article:305893 | pubmed:author | pubmed-author:RozenszajnL... | lld:pubmed |
pubmed-article:305893 | pubmed:author | pubmed-author:GoldmanII | lld:pubmed |
pubmed-article:305893 | pubmed:author | pubmed-author:ZeeviAA | lld:pubmed |
pubmed-article:305893 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:305893 | pubmed:volume | 34 | lld:pubmed |
pubmed-article:305893 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:305893 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:305893 | pubmed:pagination | 523-31 | lld:pubmed |
pubmed-article:305893 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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pubmed-article:305893 | pubmed:meshHeading | pubmed-meshheading:305893-C... | lld:pubmed |
pubmed-article:305893 | pubmed:year | 1978 | lld:pubmed |
pubmed-article:305893 | pubmed:articleTitle | Partial purification and characterization of the human lymphocyte colony enhancing factor (LCEF). | lld:pubmed |
pubmed-article:305893 | pubmed:publicationType | Journal Article | lld:pubmed |
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