| pubmed-article:3041370 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3041370 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:3041370 | lifeskim:mentions | umls-concept:C1510411 | lld:lifeskim |
| pubmed-article:3041370 | lifeskim:mentions | umls-concept:C0206691 | lld:lifeskim |
| pubmed-article:3041370 | lifeskim:mentions | umls-concept:C0013682 | lld:lifeskim |
| pubmed-article:3041370 | lifeskim:mentions | umls-concept:C0598352 | lld:lifeskim |
| pubmed-article:3041370 | pubmed:issue | 13 | lld:pubmed |
| pubmed-article:3041370 | pubmed:dateCreated | 1988-8-31 | lld:pubmed |
| pubmed-article:3041370 | pubmed:abstractText | E. coli can be transformed to extremely high efficiencies by subjecting a mixture of cells and DNA to brief but intense electrical fields of exponential decay waveform (electroporation). We have obtained 10(9) to 10(10) transformants/micrograms with strains LE392 and DH5 alpha, and plasmids pUC18 and pBR329. The process is highly dependent on two characteristics of the electrical pulse: the electric field strength and the pulse length (RC time constant). The frequency of transformation is a linear function of the DNA concentration over at least six orders of magnitude; and the efficiency of transformation is a function of the cell concentration. Most of the surviving cells are competent with up to 80% transformed at high DNA concentration. The mechanism does not appear to include binding of the DNA to the cells prior to entry. Possible mechanisms are discussed and a simple procedure for the practical use of this technique is presented. | lld:pubmed |
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| pubmed-article:3041370 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:3041370 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:3041370 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3041370 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:3041370 | pubmed:issn | 0305-1048 | lld:pubmed |
| pubmed-article:3041370 | pubmed:author | pubmed-author:MillerJ FJF | lld:pubmed |
| pubmed-article:3041370 | pubmed:author | pubmed-author:DowerW JWJ | lld:pubmed |
| pubmed-article:3041370 | pubmed:author | pubmed-author:RagsdaleC WCW | lld:pubmed |
| pubmed-article:3041370 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3041370 | pubmed:day | 11 | lld:pubmed |
| pubmed-article:3041370 | pubmed:volume | 16 | lld:pubmed |
| pubmed-article:3041370 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3041370 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3041370 | pubmed:pagination | 6127-45 | lld:pubmed |
| pubmed-article:3041370 | pubmed:dateRevised | 2010-9-9 | lld:pubmed |
| pubmed-article:3041370 | pubmed:meshHeading | pubmed-meshheading:3041370-... | lld:pubmed |
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| pubmed-article:3041370 | pubmed:meshHeading | pubmed-meshheading:3041370-... | lld:pubmed |
| pubmed-article:3041370 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:3041370 | pubmed:articleTitle | High efficiency transformation of E. coli by high voltage electroporation. | lld:pubmed |
| pubmed-article:3041370 | pubmed:affiliation | Molecular Biology Group, Bio-Rad Laboratories, Richmond, CA 94804. | lld:pubmed |
| pubmed-article:3041370 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3041370 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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