Direct extraction and assay of bone tissue collagenase and its relation to parathyroid-hormone-induced bone resorption.

Source:http://linkedlifedata.com/resource/pubmed/id/3030273

Biochem. J. 1986 Nov 1 239 3 793-6

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Authors

Eeckhout Y, Vaes G, Delaissé JM

Abstract

A method has been developed for the quantitative extraction of collagenase from as little as one 19-day-fetal-mouse calvarium. About 20-40 munits of collagenase are extracted per mg of tissue, all in a latent form that, after proper activation, shows the typical properties of mammalian collagenase. Culturing the calvaria for 2 days with parathyroid hormone (PTH) increases their procollagenase content up to 3-fold and induces bone resorption. Both PTH effects are prevented by cycloheximide, but not by indomethacin. Calcitonin inhibits resorption without affecting the PTH-induced procollagenase synthesis. The role of this synthesis is discussed in relation to the mechanisms of bone resorption.

PMID
3030273

Publication types

Research Support, Non-U.S. Gov't