| pubmed-article:3028146 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3028146 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:3028146 | lifeskim:mentions | umls-concept:C0225828 | lld:lifeskim |
| pubmed-article:3028146 | lifeskim:mentions | umls-concept:C0001492 | lld:lifeskim |
| pubmed-article:3028146 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:3028146 | lifeskim:mentions | umls-concept:C0205266 | lld:lifeskim |
| pubmed-article:3028146 | pubmed:issue | 1 Pt 1 | lld:pubmed |
| pubmed-article:3028146 | pubmed:dateCreated | 1987-3-2 | lld:pubmed |
| pubmed-article:3028146 | pubmed:abstractText | To examine the coupling of cardiac cell-surface beta-adrenergic receptors to adenylate cyclase activation and contractile response, we studied this receptor-effector response system in monolayers of spontaneously contracting chick embryo ventricular cells under physiological conditions. The hydrophilic ligand 3H-CGP12177 identified uniformly high-agonist affinity beta-adrenergic receptors (isoproterenol KD = 15 +/- 9 nM). Isoproterenol-stimulated cyclic AMP (cAMP) accumulation with 50% effective concentration at (EC50) = 12.1 nM and augmented contractile response with EC50 = 6 nM under identical conditions. One micromolar isoproterenol induced receptor loss from the cell surface with t1/2 = 13.2 min; under identical conditions cAMP content declined with t1/2 = 13.5 min and contractile response with t1/2 = 20.7 min. After agonist removal cAMP response recovered with t1/2 = 15.7 min and receptors with t1/2 = 24.7 min. Sixty minutes after agonist removal there was recovery of 52% of maximal cAMP responsiveness and 82% of the initial number of receptors; receptor occupancy was associated with 78% of initial contractile response. Agonist affinity for cell-surface receptors was changed only modestly by agonist exposure. We conclude that for this system there is relatively close coupling between high-affinity receptors, adenylate cyclase stimulation, and contractile response. | lld:pubmed |
| pubmed-article:3028146 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3028146 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3028146 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3028146 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3028146 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3028146 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3028146 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3028146 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3028146 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3028146 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3028146 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3028146 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:3028146 | pubmed:issn | 0002-9513 | lld:pubmed |
| pubmed-article:3028146 | pubmed:author | pubmed-author:RobertsD JDJ | lld:pubmed |
| pubmed-article:3028146 | pubmed:author | pubmed-author:MarshJ DJD | lld:pubmed |
| pubmed-article:3028146 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3028146 | pubmed:volume | 252 | lld:pubmed |
| pubmed-article:3028146 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3028146 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3028146 | pubmed:pagination | C47-54 | lld:pubmed |
| pubmed-article:3028146 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:3028146 | pubmed:meshHeading | pubmed-meshheading:3028146-... | lld:pubmed |
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| pubmed-article:3028146 | pubmed:meshHeading | pubmed-meshheading:3028146-... | lld:pubmed |
| pubmed-article:3028146 | pubmed:meshHeading | pubmed-meshheading:3028146-... | lld:pubmed |
| pubmed-article:3028146 | pubmed:meshHeading | pubmed-meshheading:3028146-... | lld:pubmed |
| pubmed-article:3028146 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:3028146 | pubmed:articleTitle | Adenylate cyclase regulation in intact cultured myocardial cells. | lld:pubmed |
| pubmed-article:3028146 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3028146 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:3028146 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3028146 | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3028146 | lld:pubmed |
