| pubmed-article:3009026 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3009026 | lifeskim:mentions | umls-concept:C0043393 | lld:lifeskim |
| pubmed-article:3009026 | lifeskim:mentions | umls-concept:C0014239 | lld:lifeskim |
| pubmed-article:3009026 | lifeskim:mentions | umls-concept:C0040715 | lld:lifeskim |
| pubmed-article:3009026 | lifeskim:mentions | umls-concept:C1522702 | lld:lifeskim |
| pubmed-article:3009026 | lifeskim:mentions | umls-concept:C0599718 | lld:lifeskim |
| pubmed-article:3009026 | lifeskim:mentions | umls-concept:C0599813 | lld:lifeskim |
| pubmed-article:3009026 | lifeskim:mentions | umls-concept:C0599893 | lld:lifeskim |
| pubmed-article:3009026 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:3009026 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:3009026 | pubmed:dateCreated | 1986-6-10 | lld:pubmed |
| pubmed-article:3009026 | pubmed:abstractText | The in vitro synthesized precursor of the alpha-factor pheromone, prepro-alpha-factor, of Saccharomyces cerevisiae was translocated across yeast microsomal membranes in either a homologous or a wheat germ cell free system. Translocated prepro-alpha-factor was glycosylated, sedimented with yeast microsomal vesicles, and was protected from digestion by added protease, but was soluble after alkaline sodium carbonate treatment. Thus prepro-alpha-factor was properly sequestered within yeast microsomal vesicles, but was not integrated into the lipid bilayer. In marked contrast to protein translocation across mammalian microsomal membranes, translocation of prepro-alpha-factor across yeast microsomal membranes could occur posttranslationally. This reaction required protein components in the yeast microsomal fraction that could be inactivated by alkylation or proteolysis, was ATP-dependent, and was insensitive to the presence of a variety of uncouplers and ionophores. | lld:pubmed |
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| pubmed-article:3009026 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3009026 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3009026 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3009026 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:3009026 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3009026 | pubmed:month | May | lld:pubmed |
| pubmed-article:3009026 | pubmed:issn | 0092-8674 | lld:pubmed |
| pubmed-article:3009026 | pubmed:author | pubmed-author:WalterPP | lld:pubmed |
| pubmed-article:3009026 | pubmed:author | pubmed-author:HansenWW | lld:pubmed |
| pubmed-article:3009026 | pubmed:author | pubmed-author:GarciaP DPD | lld:pubmed |
| pubmed-article:3009026 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3009026 | pubmed:day | 9 | lld:pubmed |
| pubmed-article:3009026 | pubmed:volume | 45 | lld:pubmed |
| pubmed-article:3009026 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3009026 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3009026 | pubmed:pagination | 397-406 | lld:pubmed |
| pubmed-article:3009026 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:3009026 | pubmed:meshHeading | pubmed-meshheading:3009026-... | lld:pubmed |
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| pubmed-article:3009026 | pubmed:meshHeading | pubmed-meshheading:3009026-... | lld:pubmed |
| pubmed-article:3009026 | pubmed:year | 1986 | lld:pubmed |
| pubmed-article:3009026 | pubmed:articleTitle | In vitro protein translocation across the yeast endoplasmic reticulum: ATP-dependent posttranslational translocation of the prepro-alpha-factor. | lld:pubmed |
| pubmed-article:3009026 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3009026 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:3009026 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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