pubmed-article:2993314 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:2993314 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
pubmed-article:2993314 | lifeskim:mentions | umls-concept:C0020205 | lld:lifeskim |
pubmed-article:2993314 | lifeskim:mentions | umls-concept:C1334879 | lld:lifeskim |
pubmed-article:2993314 | lifeskim:mentions | umls-concept:C0027754 | lld:lifeskim |
pubmed-article:2993314 | lifeskim:mentions | umls-concept:C0003250 | lld:lifeskim |
pubmed-article:2993314 | lifeskim:mentions | umls-concept:C0027752 | lld:lifeskim |
pubmed-article:2993314 | lifeskim:mentions | umls-concept:C0040549 | lld:lifeskim |
pubmed-article:2993314 | lifeskim:mentions | umls-concept:C1261381 | lld:lifeskim |
pubmed-article:2993314 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
pubmed-article:2993314 | lifeskim:mentions | umls-concept:C0035570 | lld:lifeskim |
pubmed-article:2993314 | pubmed:issue | 3 | lld:pubmed |
pubmed-article:2993314 | pubmed:dateCreated | 1985-10-9 | lld:pubmed |
pubmed-article:2993314 | pubmed:abstractText | A hybrid toxin composed of ricin A chain and a monoclonal antibody directed against the rat nerve growth factor (NGF) receptor (192-IgG) was prepared using the heterobifunctional cross-linking agent N-succinimidyl-3-(2-pyridyldithio)-propionate and purified by affinity chromatography. Characterization studies showed that the hybrid, 192-s-s-A, displaced bound 125I-labeled 192-IgG from rat superior cervical ganglion (SCG) membranes with an IC50 3-5 times lower than that of unconjugated 192-IgG. When incubated with cultured rat SCG neurons, 192-s-s-A inhibited protein synthesis in a concentration-dependent fashion. The effect of 192-s-s-A on these neurons was reversed by coincubation with an excess of 192-IgG. The IC50 of 192-s-s-A on protein synthesis in rat SCG neurons was 4 nM. Intact ricin and ricin A chain inhibited protein synthesis in these neurons with IC50 values of 5 pM and 500 nM, respectively. The 192-s-s-A hybrid had no effect on mouse SCG neurons or a human melanoma cell line known to have NGF receptors. This is consistent with the finding that 192-IgG recognizes only the rat NGF receptor. Also, 192-s-s-A did not inhibit protein synthesis in primary cultures of rat skeletal muscle or Vero cells, which do not have cell surface receptors for NGF. 192-s-s-A was able to inhibit protein synthesis in PC12 cells but the potency was 10-100 times less in these cells compared to rat SCG neurons. Ricin and A chain were also 10-100 times less potent in PC12 cells than neurons. Rat SCG neurons exposed to 192-s-s-A lost their refractile appearance under phase-contrast optics, showed granular degeneration of neurites, and died. Thus the decreased protein synthesis caused by the hybrid toxin correlated with the morphological destruction of the neurons. 192-s-s-A represents a potentially powerful tool by which to selectively destroy NGF receptor-bearing cells in vitro. The hybrid toxin may prove useful as an in vivo toxin. | lld:pubmed |
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pubmed-article:2993314 | pubmed:language | eng | lld:pubmed |
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pubmed-article:2993314 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:2993314 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:2993314 | pubmed:month | Sep | lld:pubmed |
pubmed-article:2993314 | pubmed:issn | 0021-9525 | lld:pubmed |
pubmed-article:2993314 | pubmed:author | pubmed-author:JohnsonE... | lld:pubmed |
pubmed-article:2993314 | pubmed:author | pubmed-author:TaniuchiMM | lld:pubmed |
pubmed-article:2993314 | pubmed:author | pubmed-author:DiStefanoP... | lld:pubmed |
pubmed-article:2993314 | pubmed:author | pubmed-author:SchweitzerJ... | lld:pubmed |
pubmed-article:2993314 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:2993314 | pubmed:volume | 101 | lld:pubmed |
pubmed-article:2993314 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:2993314 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:2993314 | pubmed:pagination | 1107-14 | lld:pubmed |
pubmed-article:2993314 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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pubmed-article:2993314 | pubmed:year | 1985 | lld:pubmed |
pubmed-article:2993314 | pubmed:articleTitle | Selective destruction of nerve growth factor receptor-bearing cells in vitro using a hybrid toxin composed of ricin A chain and a monoclonal antibody against the nerve growth factor receptor. | lld:pubmed |
pubmed-article:2993314 | pubmed:publicationType | Journal Article | lld:pubmed |
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