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| pubmed-article:2993248 | lifeskim:mentions | umls-concept:C0314829 | lld:lifeskim |
| pubmed-article:2993248 | lifeskim:mentions | umls-concept:C0079866 | lld:lifeskim |
| pubmed-article:2993248 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:2993248 | lifeskim:mentions | umls-concept:C0600401 | lld:lifeskim |
| pubmed-article:2993248 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:2993248 | lifeskim:mentions | umls-concept:C0699789 | lld:lifeskim |
| pubmed-article:2993248 | lifeskim:mentions | umls-concept:C1879748 | lld:lifeskim |
| pubmed-article:2993248 | lifeskim:mentions | umls-concept:C1706853 | lld:lifeskim |
| pubmed-article:2993248 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:2993248 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:2993248 | pubmed:dateCreated | 1985-10-23 | lld:pubmed |
| pubmed-article:2993248 | pubmed:abstractText | Suicide plasmid pJB4JI, containing transposon Tn5 and phage Mu, was introduced into Aeromonas salmonicida 449 which produces a surface protein array known as the A-layer. Kanamycin-resistant exconjugants of 449 with altered ability to produce the A-layer were selected by virtue of their altered colonial morphology and color on medium containing the dye Congo red. Analysis of culture supernatants, periplasmic shock fluid, outer membranes, and whole-cell lysates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting with a monoclonal antibody to A-protein revealed five classes of single-insertion mutations that affected the ability of cells to produce and export A-protein and to assemble the A-layer. These studies suggest that A-protein is produced from a single chromosomal gene. The subunits subsequently pass through the periplasm and across the outer membrane. At least one gene product is required for this export. Assembly of A-layer on the cell surface then requires the presence of O polysaccharide chains on the lipopolysaccharide. In one case, insertion of Tn5 resulted in loss of ability to produce both A-protein and lipopolysaccharide with O polysaccharide chains, suggesting that synthesis of A-protein and synthesis of lipopolysaccharide may involve coordinate regulation. | lld:pubmed |
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| pubmed-article:2993248 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:2993248 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2993248 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2993248 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:2993248 | pubmed:issn | 0021-9193 | lld:pubmed |
| pubmed-article:2993248 | pubmed:author | pubmed-author:TrustT JTJ | lld:pubmed |
| pubmed-article:2993248 | pubmed:author | pubmed-author:BellandR JRJ | lld:pubmed |
| pubmed-article:2993248 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2993248 | pubmed:volume | 163 | lld:pubmed |
| pubmed-article:2993248 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2993248 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2993248 | pubmed:pagination | 877-81 | lld:pubmed |
| pubmed-article:2993248 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:2993248 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:2993248 | pubmed:articleTitle | Synthesis, export, and assembly of Aeromonas salmonicida A-layer analyzed by transposon mutagenesis. | lld:pubmed |
| pubmed-article:2993248 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2993248 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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