| pubmed-article:2991858 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2991858 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:2991858 | lifeskim:mentions | umls-concept:C0023440 | lld:lifeskim |
| pubmed-article:2991858 | lifeskim:mentions | umls-concept:C1517631 | lld:lifeskim |
| pubmed-article:2991858 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:2991858 | lifeskim:mentions | umls-concept:C0005221 | lld:lifeskim |
| pubmed-article:2991858 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:2991858 | lifeskim:mentions | umls-concept:C0682002 | lld:lifeskim |
| pubmed-article:2991858 | pubmed:issue | 14 | lld:pubmed |
| pubmed-article:2991858 | pubmed:dateCreated | 1985-9-6 | lld:pubmed |
| pubmed-article:2991858 | pubmed:abstractText | K562 cells are induced by hemin to produce gamma and epsilon globin but not beta globin, although the beta globin gene is intact, and when isolated is expressed in a transient expression assay (1, 2). We have previously shown that an epsilon globin gene transferred into K562 cells is expressed and inducible (3). In this paper, we report the stable transfer of a sickle or betaS globin gene into K562 cells. Thirty-six different transformed lines were tested; 24 of 36 lines contained an intact betaS globin gene. However, using S1 nuclease, Dot blot, and Northern blotting analyses, none of these lines showed beta globin mRNA expression. These results indicate that trans acting factors are responsible for the lack of expression of the beta globin gene in K562 cells. | lld:pubmed |
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| pubmed-article:2991858 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2991858 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2991858 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2991858 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2991858 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:2991858 | pubmed:issn | 0305-1048 | lld:pubmed |
| pubmed-article:2991858 | pubmed:author | pubmed-author:BaniGG | lld:pubmed |
| pubmed-article:2991858 | pubmed:author | pubmed-author:YoungKK | lld:pubmed |
| pubmed-article:2991858 | pubmed:author | pubmed-author:Donovan-Pelus... | lld:pubmed |
| pubmed-article:2991858 | pubmed:author | pubmed-author:CubbonRR | lld:pubmed |
| pubmed-article:2991858 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2991858 | pubmed:day | 25 | lld:pubmed |
| pubmed-article:2991858 | pubmed:volume | 13 | lld:pubmed |
| pubmed-article:2991858 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2991858 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2991858 | pubmed:pagination | 5203-13 | lld:pubmed |
| pubmed-article:2991858 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:2991858 | pubmed:meshHeading | pubmed-meshheading:2991858-... | lld:pubmed |
| pubmed-article:2991858 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:2991858 | pubmed:articleTitle | Trans acting regulation of beta globin gene expression in erythroleukemia (K562) cells. | lld:pubmed |
| pubmed-article:2991858 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2991858 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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