| pubmed-article:2991844 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2991844 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:2991844 | lifeskim:mentions | umls-concept:C0001455 | lld:lifeskim |
| pubmed-article:2991844 | lifeskim:mentions | umls-concept:C0007364 | lld:lifeskim |
| pubmed-article:2991844 | lifeskim:mentions | umls-concept:C1446409 | lld:lifeskim |
| pubmed-article:2991844 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:2991844 | lifeskim:mentions | umls-concept:C0009284 | lld:lifeskim |
| pubmed-article:2991844 | pubmed:issue | 13 | lld:pubmed |
| pubmed-article:2991844 | pubmed:dateCreated | 1985-9-4 | lld:pubmed |
| pubmed-article:2991844 | pubmed:abstractText | In previous experiments, we showed that the in vivo transcription of the colicin E1 gene was dependent on cyclic AMP in adenylate cyclase-defective mutant cells of Escherichia coli (Ebina, Y. and Nakazawa, A (1983) J. Biol. Chem. 258, 7072-7078). We now show that cyclic AMP and cyclic AMP receptor protein stimulated the in vitro transcription of the gene in the presence of spermidine. As determined in DNase I protection experiments, two binding sites for the complex of cyclic AMP and the receptor protein were identified about 60 base pairs (CRP-1) and 110 base pairs (CRP-2) upstream from the transcription initiation site of the colicin E1 gene. CRP-1 had a higher affinity for the complex than that of CRP-2. Substituting an unrelated DNA sequence for CRP-2 reduced the efficiency of in vitro stimulation of the gene by cyclic AMP and the receptor protein. These potential binding sites for the cyclic AMP-cyclic AMP receptor protein complex probably participate in the stimulation of the colicin E1 gene transcription. | lld:pubmed |
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| pubmed-article:2991844 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2991844 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2991844 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2991844 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2991844 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:2991844 | pubmed:issn | 0305-1048 | lld:pubmed |
| pubmed-article:2991844 | pubmed:author | pubmed-author:NakazawaTT | lld:pubmed |
| pubmed-article:2991844 | pubmed:author | pubmed-author:MikiTT | lld:pubmed |
| pubmed-article:2991844 | pubmed:author | pubmed-author:EbinaYY | lld:pubmed |
| pubmed-article:2991844 | pubmed:author | pubmed-author:NakazawaAA | lld:pubmed |
| pubmed-article:2991844 | pubmed:author | pubmed-author:ShirabeKK | lld:pubmed |
| pubmed-article:2991844 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2991844 | pubmed:day | 11 | lld:pubmed |
| pubmed-article:2991844 | pubmed:volume | 13 | lld:pubmed |
| pubmed-article:2991844 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2991844 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2991844 | pubmed:pagination | 4687-98 | lld:pubmed |
| pubmed-article:2991844 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:2991844 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:2991844 | pubmed:articleTitle | Positive regulation of the colicin E1 gene by cyclic AMP and cyclic AMP receptor protein. | lld:pubmed |
| pubmed-article:2991844 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2991844 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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