| pubmed-article:2991321 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2991321 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2991321 | lifeskim:mentions | umls-concept:C0017689 | lld:lifeskim |
| pubmed-article:2991321 | lifeskim:mentions | umls-concept:C0030274 | lld:lifeskim |
| pubmed-article:2991321 | lifeskim:mentions | umls-concept:C0061355 | lld:lifeskim |
| pubmed-article:2991321 | lifeskim:mentions | umls-concept:C1521991 | lld:lifeskim |
| pubmed-article:2991321 | lifeskim:mentions | umls-concept:C0376315 | lld:lifeskim |
| pubmed-article:2991321 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:2991321 | pubmed:dateCreated | 1985-9-16 | lld:pubmed |
| pubmed-article:2991321 | pubmed:abstractText | The structure of human preproglucagon, as deduced from nucleotide sequencing of the preproglucagon gene, contains two glucagon-like peptides (GLP-1 and GLP-2) in the portion C-terminal to glucagon. A rabbit antiserum was raised against synthetic GLP-1-(1-19) which had 20% cross-reactivity with synthetic GLP-1 and des-Gly37-GLP-1 amide, two possible forms of the GLP-1 whole molecule, but no significant cross-reactivity with glucagon or other pancreatic peptides. Immunocytochemistry revealed that the distribution of GLP-1-(1-19) immunoreactivity followed that of glucagon-like immunoreactivity in the normal human pancreas and in two human glucagon-secreting pancreatic tumors. Chromatography of human pancreas extracts on Sephadex G-50 gave peaks of cross-reactivity at Kav values of 0.06-0.16, 0.34-0.39, 0.54-0.58 (the elution position of synthetic GLP-1), and 0.64-0.70. The concentration of immunoreactivity in the Kav 0.54-0.58 peak measured by RIA using GLP-1 or des-Gly37-GLP-1 amide as standard was 94 +/- 7 pmol/g (mean +/- SEM), while the total pancreatic glucagon content was 4.8 +/- 0.8 nmol/g. One extract of a human glucagon-secreting pancreatic tumor contained a prominent peak of GLP-1-(1-19) peptide cross-reactivity with properties identical to those of GLP-1 or des-Gly37-GLP-1 amide on gel filtration and reverse phase high pressure liquid chromatography, but another tumor contained a preponderance of cross-reactive forms of greater molecular size. Pretreatment plasma from three patients with radiological and biochemical evidence of glucagon-secreting tumors contained a peak of cross-reactivity with the chromatographic properties of intact GLP-1. The low concentrations of intact GLP-1 in normal pancreas compared with pancreatic glucagon concentrations suggest that the majority of the proglucagon is cleaved in a manner that does not produce GLP-1, as defined by its delimiting pairs of basic amino acid residues. | lld:pubmed |
| pubmed-article:2991321 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2991321 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2991321 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:2991321 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2991321 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2991321 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2991321 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2991321 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2991321 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:2991321 | pubmed:issn | 0021-972X | lld:pubmed |
| pubmed-article:2991321 | pubmed:author | pubmed-author:PolakJ MJM | lld:pubmed |
| pubmed-article:2991321 | pubmed:author | pubmed-author:BloomS RSR | lld:pubmed |
| pubmed-article:2991321 | pubmed:author | pubmed-author:BishopA EAE | lld:pubmed |
| pubmed-article:2991321 | pubmed:author | pubmed-author:GhiglioneMM | lld:pubmed |
| pubmed-article:2991321 | pubmed:author | pubmed-author:UttenthalL... | lld:pubmed |
| pubmed-article:2991321 | pubmed:author | pubmed-author:GeorgeS KSK | lld:pubmed |
| pubmed-article:2991321 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2991321 | pubmed:volume | 61 | lld:pubmed |
| pubmed-article:2991321 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2991321 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2991321 | pubmed:pagination | 472-9 | lld:pubmed |
| pubmed-article:2991321 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2991321 | pubmed:meshHeading | pubmed-meshheading:2991321-... | lld:pubmed |
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| pubmed-article:2991321 | pubmed:meshHeading | pubmed-meshheading:2991321-... | lld:pubmed |
| pubmed-article:2991321 | pubmed:meshHeading | pubmed-meshheading:2991321-... | lld:pubmed |
| pubmed-article:2991321 | pubmed:meshHeading | pubmed-meshheading:2991321-... | lld:pubmed |
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| pubmed-article:2991321 | pubmed:meshHeading | pubmed-meshheading:2991321-... | lld:pubmed |
| pubmed-article:2991321 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:2991321 | pubmed:articleTitle | Molecular forms of glucagon-like peptide-1 in human pancreas and glucagonomas. | lld:pubmed |
| pubmed-article:2991321 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2991321 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2991321 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2991321 | lld:pubmed |
