| pubmed-article:2986955 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2986955 | lifeskim:mentions | umls-concept:C1882726 | lld:lifeskim |
| pubmed-article:2986955 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:2986955 | lifeskim:mentions | umls-concept:C1704711 | lld:lifeskim |
| pubmed-article:2986955 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:2986955 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:2986955 | pubmed:dateCreated | 1985-7-10 | lld:pubmed |
| pubmed-article:2986955 | pubmed:abstractText | Binding of [125I]iodobovine GH [( 125I]iodo-bGH) to rat liver microsomes and Golgi/endosomal fractions isolated from male and female rats has been characterized. Binding of bGH to a pure somatogenic site was suggested by the finding that 50% inhibition of [125I]iodo-bGH binding required 5-130 ng bGH, rGH, or hGH/incubation, while around 500 ng rat PRL/incubation were needed to obtain the same effect. Binding of [125I]iodo-bGH to microsomes and Golgi/endosomes was time, temperature, and protein dependent. Maximal specific binding occurred at 15-16 and 15-20 h at 22 C in Golgi and microsomal membranes, respectively. Subcellular distribution studies demonstrated in the Golgi/endosomal fractions compared to the total particulate fraction, while residual microsomes devoid of Golgi/endosomal-derived components were approximately 2-fold enriched. Low levels of somatogenic receptors were detected in lysosome-enriched fractions. Removal of endogenous ligand by treating Golgi/endosomal membranes with 3 M MgCl2 increased specific binding of bGH about 2- to 3-fold. These results indicate that approximately 50% of specific somatogenic binding sites in the low density fractions represent internalized ligand-receptor complexes. The level of rat liver somatogenic receptors did not show a pronounced sex differentiation; however, an endocrine dependence of somatogenic receptor levels is suggested by the finding that livers from rats in the late stages of pregnancy had a level of somatogenic receptors exceeding that of nonpregnant rats. | lld:pubmed |
| pubmed-article:2986955 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2986955 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2986955 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:2986955 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2986955 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2986955 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2986955 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2986955 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2986955 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2986955 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2986955 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:2986955 | pubmed:issn | 0013-7227 | lld:pubmed |
| pubmed-article:2986955 | pubmed:author | pubmed-author:GustafssonJ... | lld:pubmed |
| pubmed-article:2986955 | pubmed:author | pubmed-author:AnderssonGG | lld:pubmed |
| pubmed-article:2986955 | pubmed:author | pubmed-author:NorstedtGG | lld:pubmed |
| pubmed-article:2986955 | pubmed:author | pubmed-author:HusmanBB | lld:pubmed |
| pubmed-article:2986955 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2986955 | pubmed:volume | 116 | lld:pubmed |
| pubmed-article:2986955 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2986955 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2986955 | pubmed:pagination | 2605-11 | lld:pubmed |
| pubmed-article:2986955 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2986955 | pubmed:meshHeading | pubmed-meshheading:2986955-... | lld:pubmed |
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| pubmed-article:2986955 | pubmed:meshHeading | pubmed-meshheading:2986955-... | lld:pubmed |
| pubmed-article:2986955 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:2986955 | pubmed:articleTitle | Characterization and subcellular distribution of somatogenic receptor in rat liver. | lld:pubmed |
| pubmed-article:2986955 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2986955 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2986955 | lld:pubmed |
