| pubmed-article:2985646 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2985646 | lifeskim:mentions | umls-concept:C0010825 | lld:lifeskim |
| pubmed-article:2985646 | lifeskim:mentions | umls-concept:C0003241 | lld:lifeskim |
| pubmed-article:2985646 | lifeskim:mentions | umls-concept:C1442146 | lld:lifeskim |
| pubmed-article:2985646 | lifeskim:mentions | umls-concept:C0014441 | lld:lifeskim |
| pubmed-article:2985646 | lifeskim:mentions | umls-concept:C1709793 | lld:lifeskim |
| pubmed-article:2985646 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:2985646 | pubmed:dateCreated | 1985-6-17 | lld:pubmed |
| pubmed-article:2985646 | pubmed:abstractText | An antibody capture enzyme-linked immunosorbent assay was developed for detection of immunoglobulin E antibody to cytomegalovirus (CMV-IgE). Affinity-purified anti-human IgE-coated microtiter plates were used to separate IgE from other classes of antibody in serum. Virus-specific IgE was detected by subsequent incubation with horseradish peroxidase-labeled CMV antigen and substrate. The assay was shown to be very sensitive, since in most positive sera CMV-IgE was still detected at a dilution of 1:5,000. Of 45 patients with primary CMV infection, 43 (96%) were found to produce CMV-IgE. In contrast, CMV-IgE was detected in only 4 (9%) of 44 patients with recurrent CMV infection and in 1 of 144 healthy controls. Furthermore, the level of CMV-IgE in patients with recurrent CMV infection appeared to be lower than that in patients with primary infection. Preliminary examination of successive sera suggested that CMV-IgE is produced somewhat slower than CMV-IgM and -IgA but persists for a shorter period. These results suggest that CMV-IgE may be used as an indicator of primary CMV infection. | lld:pubmed |
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| pubmed-article:2985646 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:2985646 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2985646 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2985646 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:2985646 | pubmed:issn | 0095-1137 | lld:pubmed |
| pubmed-article:2985646 | pubmed:author | pubmed-author:van der... | lld:pubmed |
| pubmed-article:2985646 | pubmed:author | pubmed-author:van LoonA MAM | lld:pubmed |
| pubmed-article:2985646 | pubmed:author | pubmed-author:van der... | lld:pubmed |
| pubmed-article:2985646 | pubmed:author | pubmed-author:HeessenF WFW | lld:pubmed |
| pubmed-article:2985646 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2985646 | pubmed:volume | 21 | lld:pubmed |
| pubmed-article:2985646 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2985646 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2985646 | pubmed:pagination | 558-61 | lld:pubmed |
| pubmed-article:2985646 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:2985646 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:2985646 | pubmed:articleTitle | Quantitation of immunoglobulin E antibody to cytomegalovirus by antibody capture enzyme-linked immunosorbent assay. | lld:pubmed |
| pubmed-article:2985646 | pubmed:publicationType | Journal Article | lld:pubmed |
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