| pubmed-article:2952897 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2952897 | lifeskim:mentions | umls-concept:C0006772 | lld:lifeskim |
| pubmed-article:2952897 | lifeskim:mentions | umls-concept:C0039063 | lld:lifeskim |
| pubmed-article:2952897 | lifeskim:mentions | umls-concept:C0001477 | lld:lifeskim |
| pubmed-article:2952897 | lifeskim:mentions | umls-concept:C0376196 | lld:lifeskim |
| pubmed-article:2952897 | lifeskim:mentions | umls-concept:C1948023 | lld:lifeskim |
| pubmed-article:2952897 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:2952897 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:2952897 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:2952897 | pubmed:dateCreated | 1987-5-28 | lld:pubmed |
| pubmed-article:2952897 | pubmed:abstractText | A high affinity Ca2+/Mg2+ ATPase has been identified and localized in synaptic membrane subfractions. This enzyme is stimulated by low concentrations of Ca2+ (less than or equal to microM) believed to approximate the range of Ca2+ in the synaptosomal cytosol (0.1 to 5.0 microM). The opiate agonist levorphanol, in a concentration-dependent fashion, inhibited Ca2+-stimulated ATP hydrolysis in lysed synaptic membranes. This inhibition was reversed by naloxone, while dextrorphan, the inactive opiate isomer, was without effect. Inhibition by levorphanol was most pronounced in a subfraction of synaptic membranes (SPM-1). The inhibition of Ca2+-stimulated ATP hydrolysis was characterized by a reduction in Vmax for Ca2+. Levorphanol pretreatment reduced the Hill coefficient (HN) of 1.5 to 0.7, suggesting cooperative interaction between the opiate receptor and the enzyme protein. Levorphanol, but not dextrorphan, also inhibited (28%) ATP-dependent Ca2+ uptake by synaptic membranes. Opiate ligand stereoisomers were tested for their effects on calmodulin stimulating of high affinity Ca2+/Mg2+ ATPase in synaptic membranes. Levorphanol (10 microM), but not the inactive stereoisomer (+)dextrorphan, significantly inhibited (35%) the calmodulin-activated Ca2+-dependent ATP hydrolysis activity in a preparation of lysed synaptic membranes. Both Ca2+-dependent and calmodulin-dependent stimulation of the enzyme in the presence of optimal concentrations of the other co-substrate were inhibited by levorphanol (35-40%) but not dextrorphan. Inhibition of ATP hydrolysis was characterized by a reduction in Vmax for both Ca2+ and calmodulin stimulation of the enzyme. Calmodulin stimulation of enzyme activity was most pronounced in SPM-1, the membrane fraction which also exhibits the maximal opiate inhibition (40%) of the Ca2+-ATPase.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:2952897 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2952897 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2952897 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2952897 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2952897 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2952897 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2952897 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2952897 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2952897 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2952897 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2952897 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2952897 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2952897 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2952897 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:2952897 | pubmed:issn | 0364-3190 | lld:pubmed |
| pubmed-article:2952897 | pubmed:author | pubmed-author:RossD HDH | lld:pubmed |
| pubmed-article:2952897 | pubmed:author | pubmed-author:CardenasH LHL | lld:pubmed |
| pubmed-article:2952897 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2952897 | pubmed:volume | 12 | lld:pubmed |
| pubmed-article:2952897 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2952897 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2952897 | pubmed:pagination | 41-8 | lld:pubmed |
| pubmed-article:2952897 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:2952897 | pubmed:meshHeading | pubmed-meshheading:2952897-... | lld:pubmed |
| pubmed-article:2952897 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:2952897 | pubmed:articleTitle | Opiates inhibit calmodulin activation of a high-affinity Ca2+-stimulated Mg2+-dependent ATPase in synaptic membranes. | lld:pubmed |
| pubmed-article:2952897 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2952897 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2952897 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
