| pubmed-article:2939294 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2939294 | lifeskim:mentions | umls-concept:C0038317 | lld:lifeskim |
| pubmed-article:2939294 | lifeskim:mentions | umls-concept:C1512505 | lld:lifeskim |
| pubmed-article:2939294 | lifeskim:mentions | umls-concept:C0040845 | lld:lifeskim |
| pubmed-article:2939294 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:2939294 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:2939294 | pubmed:dateCreated | 1986-6-12 | lld:pubmed |
| pubmed-article:2939294 | pubmed:abstractText | Membrane binding sites for peanut lectin or peanut agglutinin (PNA) were investigated in the established mammary carcinoma cell lines MCF-7, 734-B, ZR-75.1 and BT-20. The determination of PNA binding sites was performed in a flow cytometer after staining with fluorescein(FITC)-labeled PNA. It appeared that only the estrogen-sensitive cell lines exhibited PNA binding sites, whereas the hormone-insensitive cell line BT-20 was clearly negative. Steroid hormones, when administered singly to the cells in physiological concentrations (10(-9)-10(-8) M) had no effect on PNA binding expression. Only the combination of estradiol and progesterone together increased PNA binding sites. Pharmacological doses (10(-6) M) of medroxyprogesteroneacetate (MPA) and dexamethasone increased the number of binding sites, whereas retinoic acid decreased them. A preliminary characterization of the binding sites revealed that they have high capacity and moderate affinity for PNA (KD greater than 10(-7) M). FITC-PNA binding could be inhibited selectively by fetuin (greater than 10(-5) M) and by galactose (greater than 10(-2) M). Cytosol from MCF-7 cells and from some primary breast cancer specimens were able to decrease PNA binding to the surface of 734-B cells. | lld:pubmed |
| pubmed-article:2939294 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2939294 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2939294 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2939294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2939294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2939294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2939294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2939294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2939294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2939294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2939294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2939294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2939294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2939294 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2939294 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:2939294 | pubmed:issn | 0022-4731 | lld:pubmed |
| pubmed-article:2939294 | pubmed:author | pubmed-author:DapuntOO | lld:pubmed |
| pubmed-article:2939294 | pubmed:author | pubmed-author:BöckGG | lld:pubmed |
| pubmed-article:2939294 | pubmed:author | pubmed-author:DaxenbichlerG... | lld:pubmed |
| pubmed-article:2939294 | pubmed:author | pubmed-author:MartiBB | lld:pubmed |
| pubmed-article:2939294 | pubmed:author | pubmed-author:DürkenMM | lld:pubmed |
| pubmed-article:2939294 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2939294 | pubmed:volume | 24 | lld:pubmed |
| pubmed-article:2939294 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2939294 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2939294 | pubmed:pagination | 119-24 | lld:pubmed |
| pubmed-article:2939294 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:2939294 | pubmed:meshHeading | pubmed-meshheading:2939294-... | lld:pubmed |
| pubmed-article:2939294 | pubmed:meshHeading | pubmed-meshheading:2939294-... | lld:pubmed |
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| pubmed-article:2939294 | pubmed:meshHeading | pubmed-meshheading:2939294-... | lld:pubmed |
| pubmed-article:2939294 | pubmed:meshHeading | pubmed-meshheading:2939294-... | lld:pubmed |
| pubmed-article:2939294 | pubmed:meshHeading | pubmed-meshheading:2939294-... | lld:pubmed |
| pubmed-article:2939294 | pubmed:meshHeading | pubmed-meshheading:2939294-... | lld:pubmed |
| pubmed-article:2939294 | pubmed:meshHeading | pubmed-meshheading:2939294-... | lld:pubmed |
| pubmed-article:2939294 | pubmed:year | 1986 | lld:pubmed |
| pubmed-article:2939294 | pubmed:articleTitle | Influence of steroids and retinoic acid on peanut-lectin binding of human breast cancer cells. | lld:pubmed |
| pubmed-article:2939294 | pubmed:publicationType | Journal Article | lld:pubmed |
