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pubmed-article:2925646pubmed:abstractTextCalsequestrin is a high capacity low affinity Ca2+-binding protein thought to be essential for the function of the intracellular rapid releasable Ca2+ pool of a variety of animal cells. Here we show that two types of plant tissues, cultured Streptanthus tortuosus cells and spinach leaves, contain a form of calsequestrin. In subcellular fractions of S. tortuosus cells, Stains-all staining reveals a metachromatically blue-staining 56,000-Da protein enriched in the microsomal fraction. This protein shares several biochemical characteristics with animal calsequestrin: 1) it changes its apparent molecular weight with the pH; 2) it is able to bind 45Ca2+ on nitrocellulose transfers; and 3) it is recognized by antibodies against canine cardiac calsequestrin. Calsequestrin was also identified in spinach leaves using a direct extraction procedure that was developed for muscle calsequestrin. Thus, our results demonstrate that plant cells contain calsequestrin within a subcellular membrane fraction. These results also suggest that calsequestrin is an ubiquitous protein rather than being limited only to animal cells.lld:pubmed
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pubmed-article:2925646pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:2925646pubmed:articleTitlePlant cells contain calsequestrin.lld:pubmed
pubmed-article:2925646pubmed:affiliationDepartment of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City 52242.lld:pubmed
pubmed-article:2925646pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:2925646pubmed:publicationTypeComparative Studylld:pubmed
pubmed-article:2925646pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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