pubmed-article:2912138 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:2912138 | lifeskim:mentions | umls-concept:C0009368 | lld:lifeskim |
pubmed-article:2912138 | lifeskim:mentions | umls-concept:C0005528 | lld:lifeskim |
pubmed-article:2912138 | lifeskim:mentions | umls-concept:C0002502 | lld:lifeskim |
pubmed-article:2912138 | lifeskim:mentions | umls-concept:C0021467 | lld:lifeskim |
pubmed-article:2912138 | lifeskim:mentions | umls-concept:C0021469 | lld:lifeskim |
pubmed-article:2912138 | lifeskim:mentions | umls-concept:C0597484 | lld:lifeskim |
pubmed-article:2912138 | lifeskim:mentions | umls-concept:C0243071 | lld:lifeskim |
pubmed-article:2912138 | pubmed:issue | 1 Pt 1 | lld:pubmed |
pubmed-article:2912138 | pubmed:dateCreated | 1989-2-21 | lld:pubmed |
pubmed-article:2912138 | pubmed:abstractText | The potency of several amiloride analogues to inhibit electrogenic Na+ transport in colon from dexamethasone-treated rats was compared. Short-circuit current (Isc) across the colonic mucosa and 22Na+ uptake into membrane vesicles derived from colonic enterocytes was determined in dexamethasone-treated rats. Kinetic analysis of inhibition of Isc and 22Na+ uptake revealed the presence of a high- and low-affinity amiloride pathway. One pathway had a high affinity [(Ki-Isc; Ki uptake] to benzamil (15.5 nM; 5.4 nM), phenamil (19.4 nM; 7.0 nM), 3',4'-dichlorobenzamil (29.0 nM; 25.2 nM), and amiloride (115 nM; 12.4 nM) but a much lower affinity to 5-(N-ethyl-N-isopropyl)amiloride (EIPA) (greater than 100 microM; greater than 9.9 microM) and 5-(N-propyl-N-butyl)-2'-4'-dichlorobenzamil (PBDCB) (greater than microM; greater than 32.8 microM). The high-affinity pathway accounted for 75-83% of the transport of Na+. The second pathway had nearly the same low affinity for each of the analogues (e.g., amiloride Ki-Isc 1 microM; Ki uptake 4 microM) and accounted for only 15-25% of the transport of Na+. The results demonstrate that the structure-inhibitory pattern of these amiloride analogues for the high-affinity pathway is the pattern observed in other electrogenic Na+-transporting epithelia and that this pharmacological profile is preserved in membrane vesicles derived from colonic enterocytes. In addition, the potency of EIPA and benzamil to inhibit electroneutral Na+ transport across the colon from normal rats (i.e., not treated with dexamethasone) was also investigated.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
pubmed-article:2912138 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2912138 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2912138 | pubmed:language | eng | lld:pubmed |
pubmed-article:2912138 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2912138 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:2912138 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2912138 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2912138 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2912138 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2912138 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2912138 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2912138 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2912138 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:2912138 | pubmed:month | Jan | lld:pubmed |
pubmed-article:2912138 | pubmed:issn | 0002-9513 | lld:pubmed |
pubmed-article:2912138 | pubmed:author | pubmed-author:BenosD JDJ | lld:pubmed |
pubmed-article:2912138 | pubmed:author | pubmed-author:BridgesR JRJ | lld:pubmed |
pubmed-article:2912138 | pubmed:author | pubmed-author:FrizzellR ARA | lld:pubmed |
pubmed-article:2912138 | pubmed:author | pubmed-author:CragoeE JEJJr | lld:pubmed |
pubmed-article:2912138 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:2912138 | pubmed:volume | 256 | lld:pubmed |
pubmed-article:2912138 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:2912138 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:2912138 | pubmed:pagination | C67-74 | lld:pubmed |
pubmed-article:2912138 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
pubmed-article:2912138 | pubmed:meshHeading | pubmed-meshheading:2912138-... | lld:pubmed |
pubmed-article:2912138 | pubmed:meshHeading | pubmed-meshheading:2912138-... | lld:pubmed |
pubmed-article:2912138 | pubmed:meshHeading | pubmed-meshheading:2912138-... | lld:pubmed |
pubmed-article:2912138 | pubmed:meshHeading | pubmed-meshheading:2912138-... | lld:pubmed |
pubmed-article:2912138 | pubmed:meshHeading | pubmed-meshheading:2912138-... | lld:pubmed |
pubmed-article:2912138 | pubmed:meshHeading | pubmed-meshheading:2912138-... | lld:pubmed |
pubmed-article:2912138 | pubmed:meshHeading | pubmed-meshheading:2912138-... | lld:pubmed |
pubmed-article:2912138 | pubmed:meshHeading | pubmed-meshheading:2912138-... | lld:pubmed |
pubmed-article:2912138 | pubmed:meshHeading | pubmed-meshheading:2912138-... | lld:pubmed |
pubmed-article:2912138 | pubmed:meshHeading | pubmed-meshheading:2912138-... | lld:pubmed |
pubmed-article:2912138 | pubmed:meshHeading | pubmed-meshheading:2912138-... | lld:pubmed |
pubmed-article:2912138 | pubmed:meshHeading | pubmed-meshheading:2912138-... | lld:pubmed |
pubmed-article:2912138 | pubmed:meshHeading | pubmed-meshheading:2912138-... | lld:pubmed |
pubmed-article:2912138 | pubmed:year | 1989 | lld:pubmed |
pubmed-article:2912138 | pubmed:articleTitle | Inhibition of colonic Na+ transport by amiloride analogues. | lld:pubmed |
pubmed-article:2912138 | pubmed:affiliation | Department of Physiology and Biophysics, University of Alabama, Birmingham 35294. | lld:pubmed |
pubmed-article:2912138 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:2912138 | pubmed:publicationType | Comparative Study | lld:pubmed |
pubmed-article:2912138 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2912138 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2912138 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2912138 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2912138 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2912138 | lld:pubmed |