| pubmed-article:2847006 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2847006 | lifeskim:mentions | umls-concept:C0008170 | lld:lifeskim |
| pubmed-article:2847006 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:2847006 | pubmed:issue | 2-3 | lld:pubmed |
| pubmed-article:2847006 | pubmed:dateCreated | 1988-12-21 | lld:pubmed |
| pubmed-article:2847006 | pubmed:abstractText | We have constructed a small, transposition-defective derivative of the transposon Tn10 that carries the chloramphenicol acetyltransferase gene of pACYC184. This new genetic element, Tn10d-Cam, transposes when Tn10 transposase is provided from a multi-copy plasmid. Transposon insertion mutagenesis of Salmonella typhimurium was performed by using a strain carrying a Tn10d-Cam insertion in an Escherichia coli F' episome as the donor in transductional crosses into recipients that carried a plasmid expressing Tn10 transposase. Tn10d-Cam insertion mutations were also generated by complementation in cis of Tn10d-Cam by a cotransducible Tn10 element that overproduces transposase. Here, transposase was provided only transiently, and the Tn10d-Cam insertion mutations were recovered in a transposase-free strain. Cis complementation was used for mutagenesis of a plasmid target. The site specificity of insertion and the effect of insertions on expression of a downstream gene were investigated, using Tn10d-Cam insertions in a plasmid carrying a segment of the histidine operon. | lld:pubmed |
| pubmed-article:2847006 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2847006 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2847006 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2847006 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2847006 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2847006 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2847006 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:2847006 | pubmed:issn | 0026-8925 | lld:pubmed |
| pubmed-article:2847006 | pubmed:author | pubmed-author:RothJ RJR | lld:pubmed |
| pubmed-article:2847006 | pubmed:author | pubmed-author:ElliottTT | lld:pubmed |
| pubmed-article:2847006 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2847006 | pubmed:volume | 213 | lld:pubmed |
| pubmed-article:2847006 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2847006 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2847006 | pubmed:pagination | 332-8 | lld:pubmed |
| pubmed-article:2847006 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2847006 | pubmed:meshHeading | pubmed-meshheading:2847006-... | lld:pubmed |
| pubmed-article:2847006 | pubmed:meshHeading | pubmed-meshheading:2847006-... | lld:pubmed |
| pubmed-article:2847006 | pubmed:meshHeading | pubmed-meshheading:2847006-... | lld:pubmed |
| pubmed-article:2847006 | pubmed:meshHeading | pubmed-meshheading:2847006-... | lld:pubmed |
| pubmed-article:2847006 | pubmed:meshHeading | pubmed-meshheading:2847006-... | lld:pubmed |
| pubmed-article:2847006 | pubmed:meshHeading | pubmed-meshheading:2847006-... | lld:pubmed |
| pubmed-article:2847006 | pubmed:meshHeading | pubmed-meshheading:2847006-... | lld:pubmed |
| pubmed-article:2847006 | pubmed:meshHeading | pubmed-meshheading:2847006-... | lld:pubmed |
| pubmed-article:2847006 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:2847006 | pubmed:articleTitle | Characterization of Tn10d-Cam: a transposition-defective Tn10 specifying chloramphenicol resistance. | lld:pubmed |
| pubmed-article:2847006 | pubmed:affiliation | Department of Biology, University of Utah, Salt Lake City 84112. | lld:pubmed |
| pubmed-article:2847006 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2847006 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2847006 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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