| pubmed-article:2839761 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2839761 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:2839761 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:2839761 | lifeskim:mentions | umls-concept:C0806036 | lld:lifeskim |
| pubmed-article:2839761 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:2839761 | lifeskim:mentions | umls-concept:C0001492 | lld:lifeskim |
| pubmed-article:2839761 | lifeskim:mentions | umls-concept:C0030071 | lld:lifeskim |
| pubmed-article:2839761 | lifeskim:mentions | umls-concept:C0443211 | lld:lifeskim |
| pubmed-article:2839761 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:2839761 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:2839761 | pubmed:dateCreated | 1988-8-17 | lld:pubmed |
| pubmed-article:2839761 | pubmed:abstractText | The nonselective alpha-adrenergic agonist oxymetazoline inhibits parathyroid hormone (PTH)-stimulated cAMP production in intact OK cells, an epithelial cell line derived from an American opossum kidney. This inhibition, however, is not blocked by alpha 2-adrenergic receptor antagonists. After excluding several alternate hypotheses to explain this anomalous activity of oxymetazoline, we hypothesized that oxymetazoline activates a receptor in OK cells that is negatively coupled to adenylate cyclase but distinct from the alpha 2-adrenergic receptor. Prior exposure of OK cells to pertussis toxin blocks the inhibitory response to oxymetazoline, suggesting involvement of a guanine nucleotide-binding regulatory protein. Screening various compounds for attenuation of PTH-stimulated adenylate cyclase showed that serotonin (5HT) is a potent and fully efficacious agonist. Desensitization of alpha 2-receptor-mediated inhibition of cAMP production by epinephrine did not alter the response to either 5HT or oxymetazoline, indicating that these compounds do not produce their effect by activating alpha 2-adrenergic receptors. The 5HT1 receptor-selective antagonist methiothepin, but not ketanserin (5HT2-selective) or ICS-205,930 (5HT3-selective), blocked the response to both 5HT and oxymetazoline. The potency of methiothepin for antagonizing oxymetazoline-induced inhibition of PTH-stimulated cAMP production was not significantly different from its potency for the 5HT-induced effect. These data indicate that OK cells express a 5HT1 receptor that is negatively coupled to adenylate cyclase and that oxymetazoline is an agonist at these receptors. | lld:pubmed |
| pubmed-article:2839761 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2839761 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2839761 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2839761 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2839761 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2839761 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2839761 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2839761 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2839761 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2839761 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2839761 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2839761 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2839761 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2839761 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:2839761 | pubmed:issn | 0026-895X | lld:pubmed |
| pubmed-article:2839761 | pubmed:author | pubmed-author:BylundD BDB | lld:pubmed |
| pubmed-article:2839761 | pubmed:author | pubmed-author:MurphyT JTJ | lld:pubmed |
| pubmed-article:2839761 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2839761 | pubmed:volume | 34 | lld:pubmed |
| pubmed-article:2839761 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2839761 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2839761 | pubmed:pagination | 1-7 | lld:pubmed |
| pubmed-article:2839761 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2839761 | pubmed:meshHeading | pubmed-meshheading:2839761-... | lld:pubmed |
| pubmed-article:2839761 | pubmed:meshHeading | pubmed-meshheading:2839761-... | lld:pubmed |
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| pubmed-article:2839761 | pubmed:meshHeading | pubmed-meshheading:2839761-... | lld:pubmed |
| pubmed-article:2839761 | pubmed:meshHeading | pubmed-meshheading:2839761-... | lld:pubmed |
| pubmed-article:2839761 | pubmed:meshHeading | pubmed-meshheading:2839761-... | lld:pubmed |
| pubmed-article:2839761 | pubmed:meshHeading | pubmed-meshheading:2839761-... | lld:pubmed |
| pubmed-article:2839761 | pubmed:meshHeading | pubmed-meshheading:2839761-... | lld:pubmed |
| pubmed-article:2839761 | pubmed:meshHeading | pubmed-meshheading:2839761-... | lld:pubmed |
| pubmed-article:2839761 | pubmed:meshHeading | pubmed-meshheading:2839761-... | lld:pubmed |
| pubmed-article:2839761 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:2839761 | pubmed:articleTitle | Oxymetazoline inhibits adenylate cyclase by activation of serotonin-1 receptors in the OK cell, an established renal epithelial cell line. | lld:pubmed |
| pubmed-article:2839761 | pubmed:affiliation | Department of Pharmacology, School of Medicine, University of Missouri-Columbia 65212. | lld:pubmed |
| pubmed-article:2839761 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2839761 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2839761 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2839761 | lld:pubmed |
