| pubmed-article:2835484 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2835484 | lifeskim:mentions | umls-concept:C0022646 | lld:lifeskim |
| pubmed-article:2835484 | lifeskim:mentions | umls-concept:C1318418 | lld:lifeskim |
| pubmed-article:2835484 | lifeskim:mentions | umls-concept:C1621574 | lld:lifeskim |
| pubmed-article:2835484 | lifeskim:mentions | umls-concept:C0597484 | lld:lifeskim |
| pubmed-article:2835484 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:2835484 | pubmed:dateCreated | 1988-6-14 | lld:pubmed |
| pubmed-article:2835484 | pubmed:abstractText | In order to permit future characterization and possible isolation of the Na+-H+ exchanger from the apical membrane of proximal tubular cells, studies were performed to solubilize and reconstitute this transporter. Rabbit brush border membranes were prepared by a magnesium aggregation method, solubilized with the detergent octyl glucoside, and reconstituted into artificial phospholipid vesicles. In the presence of a pH gradient (pHin 6.0, pHout 8.0), the uptake of 1 mM 22Na+ into the proteoliposomes was five- to sevenfold higher than into liposomes. Amiloride (2 mM) inhibited proton gradient-stimulated uptake of sodium by 50%. As compared to proton gradient conditions, the uptake of sodium was lower in the absence of a pH gradient but was significantly higher when the outside and inside pH was 6.0 than 8.0. The Ka for sodium in reconstituted proteoliposomes studied under pH gradient conditions was 4 mM. The uptake of sodium in proteoliposomes prepared from heat-denatured membrane proteins was significantly decreased. These studies demonstrate that proteoliposomes prepared from octyl glucoside-solubilized brush border membrane proteins and asolectin exhibit proton gradient-stimulated, amiloride-inhibitable, electroneutral uptake of sodium. The ability to solubilize and reconstitute the Na+-H+ exchanger from the apical membrane of the proximal tubule will be of value in isolating and characterizing this transporter. | lld:pubmed |
| pubmed-article:2835484 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2835484 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2835484 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2835484 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2835484 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2835484 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2835484 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2835484 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2835484 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2835484 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2835484 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2835484 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2835484 | pubmed:issn | 0022-2631 | lld:pubmed |
| pubmed-article:2835484 | pubmed:author | pubmed-author:WeinmanE JEJ | lld:pubmed |
| pubmed-article:2835484 | pubmed:author | pubmed-author:DubinskyW PWP | lld:pubmed |
| pubmed-article:2835484 | pubmed:author | pubmed-author:ShenolikarSS | lld:pubmed |
| pubmed-article:2835484 | pubmed:author | pubmed-author:CragoeE JEJJr | lld:pubmed |
| pubmed-article:2835484 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2835484 | pubmed:volume | 101 | lld:pubmed |
| pubmed-article:2835484 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2835484 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2835484 | pubmed:pagination | 1-9 | lld:pubmed |
| pubmed-article:2835484 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2835484 | pubmed:meshHeading | pubmed-meshheading:2835484-... | lld:pubmed |
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| pubmed-article:2835484 | pubmed:meshHeading | pubmed-meshheading:2835484-... | lld:pubmed |
| pubmed-article:2835484 | pubmed:meshHeading | pubmed-meshheading:2835484-... | lld:pubmed |
| pubmed-article:2835484 | pubmed:meshHeading | pubmed-meshheading:2835484-... | lld:pubmed |
| pubmed-article:2835484 | pubmed:meshHeading | pubmed-meshheading:2835484-... | lld:pubmed |
| pubmed-article:2835484 | pubmed:meshHeading | pubmed-meshheading:2835484-... | lld:pubmed |
| pubmed-article:2835484 | pubmed:meshHeading | pubmed-meshheading:2835484-... | lld:pubmed |
| pubmed-article:2835484 | pubmed:meshHeading | pubmed-meshheading:2835484-... | lld:pubmed |
| pubmed-article:2835484 | pubmed:meshHeading | pubmed-meshheading:2835484-... | lld:pubmed |
| pubmed-article:2835484 | pubmed:meshHeading | pubmed-meshheading:2835484-... | lld:pubmed |
| pubmed-article:2835484 | pubmed:meshHeading | pubmed-meshheading:2835484-... | lld:pubmed |
| pubmed-article:2835484 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:2835484 | pubmed:articleTitle | Solubilization and reconstitution of renal brush border Na+-H+ exchanger. | lld:pubmed |
| pubmed-article:2835484 | pubmed:affiliation | Department of Internal Medicine, Pharmacology, and Physiology, University of Texas Medical School, Houston 77225. | lld:pubmed |
| pubmed-article:2835484 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2835484 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2835484 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2835484 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2835484 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2835484 | lld:pubmed |
