| pubmed-article:2833278 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2833278 | lifeskim:mentions | umls-concept:C1135918 | lld:lifeskim |
| pubmed-article:2833278 | lifeskim:mentions | umls-concept:C0034193 | lld:lifeskim |
| pubmed-article:2833278 | lifeskim:mentions | umls-concept:C0034826 | lld:lifeskim |
| pubmed-article:2833278 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:2833278 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:2833278 | pubmed:dateCreated | 1988-5-10 | lld:pubmed |
| pubmed-article:2833278 | pubmed:abstractText | Smooth muscle cells from the gastric antrum of the rabbit were isolated using collagenase and pronase. We examined the characteristics of muscarinic receptors that control contraction of the muscle cell: kinetics, stoichiometry and specificity of both contractile response to muscarinic agents and binding of labeled N-methyl-scopolamine. Cells contracted in the presence of muscarinic agents after a short time (30 sec) while binding of (3H)-NMS reached a plateau after 10 min exposure. Specific binding was saturable and Scatchard analysis revealed a single class of high-affinity binding sites (Kd: 0.5 nM). Oxotremorine was the most potent agonist with an ED50 of 0.6 pM; acetylcholine and carbachol were 10 times less potent. Muscarinic antagonists competed with (3H)-NMS for binding with IC50 values in the same range (nanomolar or less) than those obtained for inhibition of acetylcholine-induced contractions. Pirenzepine antagonized contractile effect of muscarinic agonists with EC50 in a micromolar range. Intracellular levels of cyclic AMP were lowered by muscarinic agonists. Monoclonal anti-muscarinic receptor antibodies M-35 displayed agonist-like activities triggering contraction and lowering cyclic AMP levels of the cells. However, although the antagonist inhibits M-35-induced contractions and cAMP decrease, M-35 had no effect on binding of the antagonist to the muscarinic receptor. These data revealed the presence of an M2-muscarinic receptor subtype involved in the contractile response of the isolated smooth muscle cell. | lld:pubmed |
| pubmed-article:2833278 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2833278 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2833278 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2833278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2833278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:2833278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:2833278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2833278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2833278 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2833278 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:2833278 | pubmed:issn | 0006-2952 | lld:pubmed |
| pubmed-article:2833278 | pubmed:author | pubmed-author:BaldJ GJG | lld:pubmed |
| pubmed-article:2833278 | pubmed:author | pubmed-author:MagousRR | lld:pubmed |
| pubmed-article:2833278 | pubmed:author | pubmed-author:StrosbergDD | lld:pubmed |
| pubmed-article:2833278 | pubmed:author | pubmed-author:MoummiCC | lld:pubmed |
| pubmed-article:2833278 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2833278 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:2833278 | pubmed:volume | 37 | lld:pubmed |
| pubmed-article:2833278 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2833278 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2833278 | pubmed:pagination | 1363-9 | lld:pubmed |
| pubmed-article:2833278 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:2833278 | pubmed:meshHeading | pubmed-meshheading:2833278-... | lld:pubmed |
| pubmed-article:2833278 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:2833278 | pubmed:articleTitle | Muscarinic receptors in isolated smooth muscle cells from gastric antrum. | lld:pubmed |
| pubmed-article:2833278 | pubmed:affiliation | Laboratoire de Biochimie des Membranes, CNRS ER 228, Faculté de Pharmacie, Montpelier, France. | lld:pubmed |
| pubmed-article:2833278 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2833278 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:2833278 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2833278 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2833278 | lld:pubmed |
