| pubmed-article:2829717 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2829717 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:2829717 | lifeskim:mentions | umls-concept:C0949782 | lld:lifeskim |
| pubmed-article:2829717 | lifeskim:mentions | umls-concept:C0028902 | lld:lifeskim |
| pubmed-article:2829717 | lifeskim:mentions | umls-concept:C0332325 | lld:lifeskim |
| pubmed-article:2829717 | lifeskim:mentions | umls-concept:C0376211 | lld:lifeskim |
| pubmed-article:2829717 | lifeskim:mentions | umls-concept:C0680242 | lld:lifeskim |
| pubmed-article:2829717 | lifeskim:mentions | umls-concept:C0243071 | lld:lifeskim |
| pubmed-article:2829717 | lifeskim:mentions | umls-concept:C0073764 | lld:lifeskim |
| pubmed-article:2829717 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:2829717 | pubmed:dateCreated | 1988-3-11 | lld:pubmed |
| pubmed-article:2829717 | pubmed:abstractText | The mechanism of action of the quinolone analogs ofloxacin and S-25930, which are unusual because of the presence of a third ring with an asymmetric carbon, was studied. Drug-resistant strains of Escherichia coli were selected by serial passage in the presence of ofloxacin, and a mutation was mapped near the gyrA gene of DNA gyrase. DNA gyrase containing the A subunit purified from this strain as compared with the isogenic wild-type strain exhibited increased resistance to ofloxacin, proving that the mutation was located in the gyrA gene. For S-25930, the S stereoisomer was more potent than the R isomer in inhibiting wild-type E. coli and DNA gyrase containing an A subunit isolated from this strain. Both isomers had decreased potency against the isogenic ofloxacin-resistant (gyrA) strain and its purified enzyme, but the S isomer remained more potent than the R isomer. These studies, using a combined genetic and biochemical approach, demonstrate (i) that DNA gyrase is a target of the tricyclics ofloxacin and S-25930, (ii) that serial exposure to ofloxacin can select resistance to tricyclic quinolone agents by mutation in the gyrA gene, and (iii) that the more potent antibacterial activity of S relative to R S-25930 correlates with increased activity against DNA gyrase for both wild-type and ofloxacin-resistant (gyrA) isogenic strains. | lld:pubmed |
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| pubmed-article:2829717 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2829717 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2829717 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2829717 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2829717 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:2829717 | pubmed:issn | 0066-4804 | lld:pubmed |
| pubmed-article:2829717 | pubmed:author | pubmed-author:SwartzM NMN | lld:pubmed |
| pubmed-article:2829717 | pubmed:author | pubmed-author:McHughG LGL | lld:pubmed |
| pubmed-article:2829717 | pubmed:author | pubmed-author:HooperD CDC | lld:pubmed |
| pubmed-article:2829717 | pubmed:author | pubmed-author:WolfsonJ SJS | lld:pubmed |
| pubmed-article:2829717 | pubmed:author | pubmed-author:SG MGM | lld:pubmed |
| pubmed-article:2829717 | pubmed:author | pubmed-author:SouzaK SKS | lld:pubmed |
| pubmed-article:2829717 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2829717 | pubmed:volume | 31 | lld:pubmed |
| pubmed-article:2829717 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2829717 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2829717 | pubmed:pagination | 1861-3 | lld:pubmed |
| pubmed-article:2829717 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:2829717 | pubmed:meshHeading | pubmed-meshheading:2829717-... | lld:pubmed |
| pubmed-article:2829717 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:2829717 | pubmed:articleTitle | Antagonism of wild-type and resistant Escherichia coli and its DNA gyrase by the tricyclic 4-quinolone analogs ofloxacin and S-25930 stereoisomers. | lld:pubmed |
| pubmed-article:2829717 | pubmed:affiliation | Medical Services, Massachusetts General Hospital, Boston 02114. | lld:pubmed |
| pubmed-article:2829717 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2829717 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2829717 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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