| pubmed-article:2827525 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2827525 | lifeskim:mentions | umls-concept:C0026820 | lld:lifeskim |
| pubmed-article:2827525 | lifeskim:mentions | umls-concept:C0007412 | lld:lifeskim |
| pubmed-article:2827525 | lifeskim:mentions | umls-concept:C0001122 | lld:lifeskim |
| pubmed-article:2827525 | lifeskim:mentions | umls-concept:C1704632 | lld:lifeskim |
| pubmed-article:2827525 | lifeskim:mentions | umls-concept:C0871261 | lld:lifeskim |
| pubmed-article:2827525 | lifeskim:mentions | umls-concept:C0205216 | lld:lifeskim |
| pubmed-article:2827525 | lifeskim:mentions | umls-concept:C2911692 | lld:lifeskim |
| pubmed-article:2827525 | lifeskim:mentions | umls-concept:C1706817 | lld:lifeskim |
| pubmed-article:2827525 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:2827525 | pubmed:issue | 1 Pt 2 | lld:pubmed |
| pubmed-article:2827525 | pubmed:dateCreated | 1988-2-25 | lld:pubmed |
| pubmed-article:2827525 | pubmed:abstractText | To examine mechanisms of diminished contractile response to catecholamines during acidosis, we studied contractile properties, beta-adrenergic receptor properties, and intracellular pH of intact, cultured myocardial cells from chick embryo ventricle at pH 7.4 and 6.8. Contractile response was measured with an optical-video system. On changing the superfusing buffer from pH 7.4 to 6.8 there was a decline in contractile amplitude to 80% of control by 20 min. Fluorimetrically determined intracellular pH declined over a similar time course from 7.11 +/- 0.05 to 6.96 +/- 0.07 (P less than 0.05). After 45 min at pH 6.8 the contractile response to 1 microM isoproterenol was less than half of the response at pH 7.4. Antagonist and agonist ligand-binding properties of the beta-adrenergic receptor were determined in the intact cells under conditions identical to those for the contractility studies. With the use of the hydrophilic antagonist [3H]CGP-12177 that selectively labels cell-surface receptors, agonist competition studies demonstrated that acidosis had no significant effect on antagonist or agonist affinity but decreased beta-receptor number from 21 +/- 3 to 11 +/- 3 fmol/mg protein (P less than 0.02). It is probable that a decline in the number of beta-receptors on the cell surface contributes to contractile hyporesponsiveness to catecholamines during acidosis. | lld:pubmed |
| pubmed-article:2827525 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2827525 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2827525 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2827525 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2827525 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2827525 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2827525 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2827525 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2827525 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2827525 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2827525 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2827525 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2827525 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:2827525 | pubmed:issn | 0002-9513 | lld:pubmed |
| pubmed-article:2827525 | pubmed:author | pubmed-author:MarshJ DJD | lld:pubmed |
| pubmed-article:2827525 | pubmed:author | pubmed-author:KimDD | lld:pubmed |
| pubmed-article:2827525 | pubmed:author | pubmed-author:MargolisT ITI | lld:pubmed |
| pubmed-article:2827525 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2827525 | pubmed:volume | 254 | lld:pubmed |
| pubmed-article:2827525 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2827525 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2827525 | pubmed:pagination | H20-7 | lld:pubmed |
| pubmed-article:2827525 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2827525 | pubmed:meshHeading | pubmed-meshheading:2827525-... | lld:pubmed |
| pubmed-article:2827525 | pubmed:meshHeading | pubmed-meshheading:2827525-... | lld:pubmed |
| pubmed-article:2827525 | pubmed:meshHeading | pubmed-meshheading:2827525-... | lld:pubmed |
| pubmed-article:2827525 | pubmed:meshHeading | pubmed-meshheading:2827525-... | lld:pubmed |
| pubmed-article:2827525 | pubmed:meshHeading | pubmed-meshheading:2827525-... | lld:pubmed |
| pubmed-article:2827525 | pubmed:meshHeading | pubmed-meshheading:2827525-... | lld:pubmed |
| pubmed-article:2827525 | pubmed:meshHeading | pubmed-meshheading:2827525-... | lld:pubmed |
| pubmed-article:2827525 | pubmed:meshHeading | pubmed-meshheading:2827525-... | lld:pubmed |
| pubmed-article:2827525 | pubmed:meshHeading | pubmed-meshheading:2827525-... | lld:pubmed |
| pubmed-article:2827525 | pubmed:meshHeading | pubmed-meshheading:2827525-... | lld:pubmed |
| pubmed-article:2827525 | pubmed:meshHeading | pubmed-meshheading:2827525-... | lld:pubmed |
| pubmed-article:2827525 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:2827525 | pubmed:articleTitle | Mechanism of diminished contractile response to catecholamines during acidosis. | lld:pubmed |
| pubmed-article:2827525 | pubmed:affiliation | Cardiovascular Division, Brigham and Women's Hospital, Boston, Massachusetts. | lld:pubmed |
| pubmed-article:2827525 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2827525 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2827525 | lld:pubmed |
