| pubmed-article:2824612 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2824612 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:2824612 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:2824612 | lifeskim:mentions | umls-concept:C0026473 | lld:lifeskim |
| pubmed-article:2824612 | lifeskim:mentions | umls-concept:C0024432 | lld:lifeskim |
| pubmed-article:2824612 | lifeskim:mentions | umls-concept:C0038952 | lld:lifeskim |
| pubmed-article:2824612 | lifeskim:mentions | umls-concept:C1511938 | lld:lifeskim |
| pubmed-article:2824612 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:2824612 | pubmed:dateCreated | 1988-1-12 | lld:pubmed |
| pubmed-article:2824612 | pubmed:abstractText | The role of mononuclear phagocyte-specific colony-stimulating factor (CSF-1) in human monocyte to macrophage differentiation was investigated. The addition of 1000 U/ml of CSF-1 to serum-free monocyte cultures resulted in monocyte survival comparable to that in cultures containing 5% AB serum, whereas cells in serum- and CSF-1-free medium lost their viability in 3 to 5 days. The requirement for CSF-1 coincided with the time (40 to 64 hr of culture) when the major changes in morphology and biochemical function took place in monocytes undergoing differentiation into macrophages. If CSF-1 was removed from the cultures before this time, death of the monocytes resulted. In cultures containing CSF-1, as in serum containing cultures, the lysosomal enzyme acid phosphatase was enhanced 10- to 20-fold by day 4 to 5. Superoxide production in response to phorbol myristic acetate was maintained in CSF-1 cultured monocytes, but declined with time in monocytes cultured in serum. The expression of monocyte-macrophage antigens p150.95 (LeuM5), OKM1, LeuM3, Fc receptors (32.2), and HLA-DR had increased in CSF-1 containing cultures at day 4. When antigen expression was analyzed at day 2 to 3, when cell size and 90 degrees scatter characteristics were still identical to control serum-free cultures, only p150.95, HLA-DR and FcR expression were enhanced by CSF-1. Low amounts of lipopolysaccharide (0.1 ng/ml) were found to enhance monocyte survival in the absence of added CSF-1. Lipopolysaccharide-containing cultures were found to produce CSF-1 (up to 450 U/ml, as detected by radioimmunoassay). Lipopolysaccharide (1 microgram/ml), however, did not induce enhanced expression of the maturation-related antigens. Based on these observations we conclude that CSF-1 is enhancing human monocyte survival and is involved in the events leading to the differentiation of monocytes into macrophages. | lld:pubmed |
| pubmed-article:2824612 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2824612 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:2824612 | pubmed:citationSubset | AIM | lld:pubmed |
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| pubmed-article:2824612 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2824612 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:2824612 | pubmed:issn | 0022-1767 | lld:pubmed |
| pubmed-article:2824612 | pubmed:author | pubmed-author:BeckerSS | lld:pubmed |
| pubmed-article:2824612 | pubmed:author | pubmed-author:HaskillSS | lld:pubmed |
| pubmed-article:2824612 | pubmed:author | pubmed-author:WarrenM KMK | lld:pubmed |
| pubmed-article:2824612 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2824612 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:2824612 | pubmed:volume | 139 | lld:pubmed |
| pubmed-article:2824612 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2824612 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2824612 | pubmed:pagination | 3703-9 | lld:pubmed |
| pubmed-article:2824612 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:2824612 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:2824612 | pubmed:articleTitle | Colony-stimulating factor-induced monocyte survival and differentiation into macrophages in serum-free cultures. | lld:pubmed |
| pubmed-article:2824612 | pubmed:affiliation | Environmental Monitoring and Services Inc., Chapel Hill, NC 27514. | lld:pubmed |
| pubmed-article:2824612 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2824612 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2824612 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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