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pubmed-article:2820819pubmed:abstractTextBiologically active phorbol esters or a diacylglycerol induce mouse eggs to modify the zona pellucida such that sperm receptor activity is retained but the ability of bound sperm to undergo a complete acrosome reaction is lost (Y. Endo, R.M. Schultz, and G.S. Kopf. 1987. Dev. Biol. 119, 199-209). We now show that purified ZP3 from 12-O-tetradecanoyl phorbol-13-acetate (TPA)-treated eggs possesses full sperm receptor activity but has lost its ability to induce a complete acrosome reaction. The modification of the acrosome reaction-inducing activity of ZP3 from these TPA-treated eggs differs from ZP3 isolated from two-cell embryos, which cannot initiate the acrosome reaction. These results demonstrate the dissociation of the two biological activities of ZP3 and may provide a system to assess the components of ZP3 involved in the acrosome reaction.lld:pubmed
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pubmed-article:2820819pubmed:volume123lld:pubmed
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pubmed-article:2820819pubmed:pagination574-7lld:pubmed
pubmed-article:2820819pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:2820819pubmed:year1987lld:pubmed
pubmed-article:2820819pubmed:articleTitleEffects of a phorbol ester on mouse eggs: dissociation of sperm receptor activity from acrosome reaction-inducing activity of the mouse zona pellucida protein, ZP3.lld:pubmed
pubmed-article:2820819pubmed:affiliationDepartment of Obstetrics and Gynecology, School of Medicine, University of Pennsylvania, Philadelphia 19104.lld:pubmed
pubmed-article:2820819pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:2820819pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
pubmed-article:2820819pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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