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pubmed-article:2790961pubmed:abstractTextWe describe a new role for fatty acylation. Conditions were established under which vesicular transport from the cis to the medial Golgi compartment in vitro depends strongly upon the addition of a fatty acyl-coenzyme A, e.g., palmitoyl-CoA. Using an inhibitor of long-chain acyl-CoA synthetase, we demonstrate that the fatty acid has to be activated by CoA to stimulate transport. A nonhydrolyzable analog of palmitoyl-CoA competitively inhibits transport. Electron microscopy and biochemical studies show that fatty acyl-CoA is required for budding of (non-clathrin-) coated transport vesicles from Golgi cisternae and that budding is inhibited by the nonhydrolyzable analog.lld:pubmed
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pubmed-article:2790961pubmed:articleTitleFatty acyl-coenzyme A is required for budding of transport vesicles from Golgi cisternae.lld:pubmed
pubmed-article:2790961pubmed:affiliationDepartment of Biology, Princeton University, New Jersey 08544-1014.lld:pubmed
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