| pubmed-article:2784433 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2784433 | lifeskim:mentions | umls-concept:C0376604 | lld:lifeskim |
| pubmed-article:2784433 | lifeskim:mentions | umls-concept:C0521428 | lld:lifeskim |
| pubmed-article:2784433 | lifeskim:mentions | umls-concept:C0015283 | lld:lifeskim |
| pubmed-article:2784433 | lifeskim:mentions | umls-concept:C0332120 | lld:lifeskim |
| pubmed-article:2784433 | lifeskim:mentions | umls-concept:C0024471 | lld:lifeskim |
| pubmed-article:2784433 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:2784433 | pubmed:dateCreated | 1989-5-11 | lld:pubmed |
| pubmed-article:2784433 | pubmed:abstractText | The MgATP dependency of secretion was investigated in digitonin-permeabilized adrenal chromaffin cells. Shortly after permeabilization there is a component of Ca2+-dependent secretion that occurs in the absence of MgATP in the medium. This secretion occurs from cells which are permeable to Ca2+/[ethylene-bis(oxyethylenenitrilo)]tetraacetic acid buffers, to nucleotides, and to proteins. It is prevented by treatment of cells with metabolic inhibitors to reduce cellular ATP prior to permeabilization. The rate of MgATP-independent secretion is rapid and terminates by approximately 2 min after introduction of Ca2+. MgATP-independent secretion is labile and is lost unless Ca2+ is introduced within 8 min of permeabilization. MgATP-dependent secretion occurs at a slower rate than MgATP-independent secretion and continues at a constant rate for 12 min. Preincubation of permeabilized cells with MgATP enhances Ca2+-dependent secretion during a subsequent incubation in the absence of MgATP. Similar MgATP sensitivities are observed when MgATP is present only prior to or only during stimulation with Ca2+ with half-maximal stimulation occurring at 0.4-0.5 and 0.6 mM MgATP, respectively. The data indicate that intact cells are primed by intracellular ATP so that immediately upon permeabilization, there is a component of secretion which is independent of medium MgATP. MgATP partially maintains the primed state after permeabilization by acting before Ca2+ in the secretory pathway. | lld:pubmed |
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| pubmed-article:2784433 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2784433 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2784433 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2784433 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2784433 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:2784433 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:2784433 | pubmed:author | pubmed-author:HolzR WRW | lld:pubmed |
| pubmed-article:2784433 | pubmed:author | pubmed-author:BittnerM AMA | lld:pubmed |
| pubmed-article:2784433 | pubmed:author | pubmed-author:SenterR ARA | lld:pubmed |
| pubmed-article:2784433 | pubmed:author | pubmed-author:EberhardD ADA | lld:pubmed |
| pubmed-article:2784433 | pubmed:author | pubmed-author:PeppersS CSC | lld:pubmed |
| pubmed-article:2784433 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2784433 | pubmed:day | 5 | lld:pubmed |
| pubmed-article:2784433 | pubmed:volume | 264 | lld:pubmed |
| pubmed-article:2784433 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2784433 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2784433 | pubmed:pagination | 5412-9 | lld:pubmed |
| pubmed-article:2784433 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2784433 | pubmed:meshHeading | pubmed-meshheading:2784433-... | lld:pubmed |
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| pubmed-article:2784433 | pubmed:meshHeading | pubmed-meshheading:2784433-... | lld:pubmed |
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| pubmed-article:2784433 | pubmed:meshHeading | pubmed-meshheading:2784433-... | lld:pubmed |
| pubmed-article:2784433 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2784433 | pubmed:articleTitle | MgATP-independent and MgATP-dependent exocytosis. Evidence that MgATP primes adrenal chromaffin cells to undergo exocytosis. | lld:pubmed |
| pubmed-article:2784433 | pubmed:affiliation | Department of Pharmacology, University of Michigan Medical School, Ann Arbor 48109-0626. | lld:pubmed |
| pubmed-article:2784433 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2784433 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2784433 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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