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pubmed-article:2762667pubmed:abstractTextCultured renal collecting duct cells from neonatal rabbit kidney were used to examine the influence of aldosterone on enzymatic activity of citrate synthase during increase in Na+ transport. Control epithelia showed citrate synthase activity of 71 +/- 3 mU/mg protein (n = 28), while after aldosterone treatment citrate synthase activity was significantly increased to 79 +/- 6 mU/mg at 1 h (n = 5), to 88 +/- 6 mU/mg at 2 h (n = 6) and to 93 +/- 8 mU/mg protein at 3 h (n = 5). Citrate synthase activity subsequently decreased to basal values. Spironolactone fully blocked the aldosterone-induced increase in citrate synthase activity. The time course of enzyme stimulation after aldosterone administration indicates that the hormone activates citrate synthase during the physiological early response phase.lld:pubmed
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pubmed-article:2762667pubmed:articleTitleAction of aldosterone on citrate synthase in cultured renal collecting duct cells.lld:pubmed
pubmed-article:2762667pubmed:affiliationDepartment of Anatomy and Cell Biology, University of Heidelberg, FRG.lld:pubmed
pubmed-article:2762667pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:2762667pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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