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pubmed-article:2752606pubmed:abstractTextA simple monolayer invasion assay (MIA) was recently developed using confluent fibroblastic cells as a target and variants of the BW5147 murine T-cell lymphoma as invading cells. Metastatic variants were consistently invasive in the MIA whereas non-metastatic cells were not. In this paper it is reported that pertussis toxin (PT) treatment of a highly metastatic and invasive variant caused a marked delay of invasion in the MIA at concentrations from 50 pg/ml upwards. Surprisingly, PT treatment of the non-metastatic, non-invasive parental BW5147 cells induced a moderate but significant level of invasion. Morphometric analysis showed that PT provoked an increased pseudopodal activity in cells in which it also caused increased invasive potential, and a decreased motility in cells with decreased invasiveness. This finding strengthens the perception that invasive potential and the capability to perform shape changes are related characteristics in these lymphoma cells.lld:pubmed
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pubmed-article:2752606pubmed:authorpubmed-author:Van HeckeDDlld:pubmed
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pubmed-article:2752606pubmed:volume7lld:pubmed
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pubmed-article:2752606pubmed:pagination541-55lld:pubmed
pubmed-article:2752606pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:2752606pubmed:articleTitleDual effects of pertussis toxin on in vitro invasive behavior of metastatic lymphoma variants.lld:pubmed
pubmed-article:2752606pubmed:affiliationPasteur Instituut van Brabant, Brussels, Belgium.lld:pubmed
pubmed-article:2752606pubmed:publicationTypeJournal Articlelld:pubmed
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pubmed-article:2752606pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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