| pubmed-article:2748598 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2748598 | lifeskim:mentions | umls-concept:C0524841 | lld:lifeskim |
| pubmed-article:2748598 | lifeskim:mentions | umls-concept:C0282188 | lld:lifeskim |
| pubmed-article:2748598 | pubmed:issue | 14 | lld:pubmed |
| pubmed-article:2748598 | pubmed:dateCreated | 1989-8-16 | lld:pubmed |
| pubmed-article:2748598 | pubmed:abstractText | Progress in archaebacterial molecular biology requires tools for genetic analysis. We describe vectors that can be selected and maintained in either Halobacterium volcanii or Escherichia coli. A genetic determinant for resistance to the 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor mevinolin was isolated by "shotgun cloning" into a derivative of the endogenous H. volcanii plasmid pHV2, to form pWL2, which transforms sensitive H. volcanii to mevinolin resistance at high frequency. The resistance determinant, portions of pHV2, and an ampicillin- and tetracycline-resistance-conferring pBR322 derivative, pAT153, were ligated together to form the shuttle vectors pWL101 and pWL102. We describe conditions for the use of these vectors and provide preliminary definition of regions essential for drug resistance and for plasmid replication and maintenance. | lld:pubmed |
| pubmed-article:2748598 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2748598 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2748598 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2748598 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2748598 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2748598 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2748598 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2748598 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2748598 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2748598 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2748598 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2748598 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2748598 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2748598 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2748598 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2748598 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2748598 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2748598 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2748598 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2748598 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2748598 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2748598 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2748598 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2748598 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:2748598 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:2748598 | pubmed:author | pubmed-author:DoolittleW... | lld:pubmed |
| pubmed-article:2748598 | pubmed:author | pubmed-author:LamW LWL | lld:pubmed |
| pubmed-article:2748598 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2748598 | pubmed:volume | 86 | lld:pubmed |
| pubmed-article:2748598 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2748598 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2748598 | pubmed:pagination | 5478-82 | lld:pubmed |
| pubmed-article:2748598 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:2748598 | pubmed:meshHeading | pubmed-meshheading:2748598-... | lld:pubmed |
| pubmed-article:2748598 | pubmed:meshHeading | pubmed-meshheading:2748598-... | lld:pubmed |
| pubmed-article:2748598 | pubmed:meshHeading | pubmed-meshheading:2748598-... | lld:pubmed |
| pubmed-article:2748598 | pubmed:meshHeading | pubmed-meshheading:2748598-... | lld:pubmed |
| pubmed-article:2748598 | pubmed:meshHeading | pubmed-meshheading:2748598-... | lld:pubmed |
| pubmed-article:2748598 | pubmed:meshHeading | pubmed-meshheading:2748598-... | lld:pubmed |
| pubmed-article:2748598 | pubmed:meshHeading | pubmed-meshheading:2748598-... | lld:pubmed |
| pubmed-article:2748598 | pubmed:meshHeading | pubmed-meshheading:2748598-... | lld:pubmed |
| pubmed-article:2748598 | pubmed:meshHeading | pubmed-meshheading:2748598-... | lld:pubmed |
| pubmed-article:2748598 | pubmed:meshHeading | pubmed-meshheading:2748598-... | lld:pubmed |
| pubmed-article:2748598 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2748598 | pubmed:articleTitle | Shuttle vectors for the archaebacterium Halobacterium volcanii. | lld:pubmed |
| pubmed-article:2748598 | pubmed:affiliation | Department of Biochemistry, Dalhousie University, Halifax, Canada. | lld:pubmed |
| pubmed-article:2748598 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2748598 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:2748598 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2748598 | lld:pubmed |
