| pubmed-article:2721369 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2721369 | lifeskim:mentions | umls-concept:C0079441 | lld:lifeskim |
| pubmed-article:2721369 | lifeskim:mentions | umls-concept:C1512977 | lld:lifeskim |
| pubmed-article:2721369 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:2721369 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:2721369 | lifeskim:mentions | umls-concept:C1522073 | lld:lifeskim |
| pubmed-article:2721369 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:2721369 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:2721369 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:2721369 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:2721369 | pubmed:dateCreated | 1989-7-5 | lld:pubmed |
| pubmed-article:2721369 | pubmed:abstractText | Recombinant DNA plasmids coding for transforming growth factor beta 2 (TGF-beta 2) precursor and a hybrid TGF-beta 1(NH2)/beta 2(COOH) molecule consisting of the amino-terminal precursor portion of transforming growth factor-beta 1 (TGF-beta 1) linked in phase to the carboxyl terminus of mature TGF-beta 2 were constructed and transfected into COS cells. Both plasmids directed the synthesis of active TGF-beta 2 which was secreted into the supernatants of transfected cells. The TGF-beta 2 was secreted in a latent form, as an acidification step was required to demonstrate optimal biological activity. Using site-specific anti-peptide antibodies, we show that precursor and mature forms of TGF-beta 2 are produced. A stable Chinese hamster ovary (CHO) cell line expressing the hybrid TGF-beta 1(NH2)/beta 2(COOH) protein was isolated. This cell line secreted both precursor and mature forms of TGF-beta 1(NH2)/beta 2(COOH); acidification was required to demonstrate biological activity. Protein sequence analysis of recombinant TGF-beta 2 produced by this CHO clone demonstrated that correct proteolytic cleavage had occurred, suggesting that the processing signals contained within the TGF-beta 1 amino portion can function in producing mature TGF-beta 2. Receptor binding studies showed that TGF-beta 2 specifically bound predominantly to type III receptors on the surface of human palatal mesenchymal cells. The availability of active TGF-beta 2 should aid in determining its potential therapeutic use as a growth modulator. | lld:pubmed |
| pubmed-article:2721369 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2721369 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2721369 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2721369 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2721369 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2721369 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2721369 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2721369 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2721369 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:2721369 | pubmed:issn | 0198-0238 | lld:pubmed |
| pubmed-article:2721369 | pubmed:author | pubmed-author:LinKK | lld:pubmed |
| pubmed-article:2721369 | pubmed:author | pubmed-author:PurchioA FAF | lld:pubmed |
| pubmed-article:2721369 | pubmed:author | pubmed-author:WebbN RNR | lld:pubmed |
| pubmed-article:2721369 | pubmed:author | pubmed-author:MadisenLL | lld:pubmed |
| pubmed-article:2721369 | pubmed:author | pubmed-author:LioubinM NMN | lld:pubmed |
| pubmed-article:2721369 | pubmed:author | pubmed-author:FarrandA LAL | lld:pubmed |
| pubmed-article:2721369 | pubmed:author | pubmed-author:KnoxL BLB | lld:pubmed |
| pubmed-article:2721369 | pubmed:author | pubmed-author:MarzowskiJJ | lld:pubmed |
| pubmed-article:2721369 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2721369 | pubmed:volume | 8 | lld:pubmed |
| pubmed-article:2721369 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2721369 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2721369 | pubmed:pagination | 205-12 | lld:pubmed |
| pubmed-article:2721369 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:2721369 | pubmed:meshHeading | pubmed-meshheading:2721369-... | lld:pubmed |
| pubmed-article:2721369 | pubmed:meshHeading | pubmed-meshheading:2721369-... | lld:pubmed |
| pubmed-article:2721369 | pubmed:meshHeading | pubmed-meshheading:2721369-... | lld:pubmed |
| pubmed-article:2721369 | pubmed:meshHeading | pubmed-meshheading:2721369-... | lld:pubmed |
| pubmed-article:2721369 | pubmed:meshHeading | pubmed-meshheading:2721369-... | lld:pubmed |
| pubmed-article:2721369 | pubmed:meshHeading | pubmed-meshheading:2721369-... | lld:pubmed |
| pubmed-article:2721369 | pubmed:meshHeading | pubmed-meshheading:2721369-... | lld:pubmed |
| pubmed-article:2721369 | pubmed:meshHeading | pubmed-meshheading:2721369-... | lld:pubmed |
| pubmed-article:2721369 | pubmed:meshHeading | pubmed-meshheading:2721369-... | lld:pubmed |
| pubmed-article:2721369 | pubmed:meshHeading | pubmed-meshheading:2721369-... | lld:pubmed |
| pubmed-article:2721369 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2721369 | pubmed:articleTitle | Expression and characterization of recombinant TGF-beta 2 proteins produced in mammalian cells. | lld:pubmed |
| pubmed-article:2721369 | pubmed:affiliation | Department of Entomology, Texas A&M University, College Station 77843. | lld:pubmed |
| pubmed-article:2721369 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2721369 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2721369 | lld:pubmed |
