| pubmed-article:2719911 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2719911 | lifeskim:mentions | umls-concept:C1335439 | lld:lifeskim |
| pubmed-article:2719911 | lifeskim:mentions | umls-concept:C0022702 | lld:lifeskim |
| pubmed-article:2719911 | lifeskim:mentions | umls-concept:C0231448 | lld:lifeskim |
| pubmed-article:2719911 | lifeskim:mentions | umls-concept:C1707455 | lld:lifeskim |
| pubmed-article:2719911 | lifeskim:mentions | umls-concept:C0441587 | lld:lifeskim |
| pubmed-article:2719911 | lifeskim:mentions | umls-concept:C0205549 | lld:lifeskim |
| pubmed-article:2719911 | lifeskim:mentions | umls-concept:C0146894 | lld:lifeskim |
| pubmed-article:2719911 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:2719911 | pubmed:dateCreated | 1989-6-27 | lld:pubmed |
| pubmed-article:2719911 | pubmed:abstractText | The effect of alkyl group size on ability to act as deoxythymidine triphosphate (dTTP) has been studied for the carcinogen products O2-methyl-, O2-ethyl-, and O2-isopropyl-dTTP by using three types of nucleic acids as template and DNA polymerase I (Pol I) or Klenow fragment as the polymerizing enzymes. Apparent Km and relative Vmax values were determined in primer extension on M13 DNA at a single defined site, in poly[d(A-T)], and in nicked DNA. These data are the basis for calculation of the relative rate of insertion opposite A, relative to dTTP. The insertion rate for any O2-alkyl-dTTP is much higher than for a mismatch between unmodified dNTPs. Unexpectedly, O2-isopropyl-dTTP is more efficiently utilized than O2-methyl-dTTP or O2-ethyl-dTTP on each of the templates. O2-isopropyl-dTTP also substitutes for dTTP over extended times of DNA synthesis at a rate only slightly lower than that of dTTP. Parallel experiments using O4-methyl-dTTP under the same conditions show that it is incorporated opposite A more frequently than is O2-methyl-dTTP. Therefore, both the ring position and the size of the alkyl group influence polymerase recognition. Once formed, all O2-alkyl-T.A termini permit elongation, as does O4-methyl-T.A. In contrast to the relative difficulty of incorporating the O-alkyl-dTTPs, formation of the following normal base pair (C.G) occurs rapidly when dGTP is present. This indicates that a single O-alkyl-T.A pair does not confer significant structural distortion recognized by Pol I. | lld:pubmed |
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| pubmed-article:2719911 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2719911 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2719911 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2719911 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2719911 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2719911 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2719911 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2719911 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:2719911 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2719911 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2719911 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2719911 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:2719911 | pubmed:issn | 0006-2960 | lld:pubmed |
| pubmed-article:2719911 | pubmed:author | pubmed-author:SingerBB | lld:pubmed |
| pubmed-article:2719911 | pubmed:author | pubmed-author:Ku?mierekJ... | lld:pubmed |
| pubmed-article:2719911 | pubmed:author | pubmed-author:ChavezFF | lld:pubmed |
| pubmed-article:2719911 | pubmed:author | pubmed-author:GoodmanM FMF | lld:pubmed |
| pubmed-article:2719911 | pubmed:author | pubmed-author:SpenglerS JSJ | lld:pubmed |
| pubmed-article:2719911 | pubmed:author | pubmed-author:MendelmanLL | lld:pubmed |
| pubmed-article:2719911 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2719911 | pubmed:day | 21 | lld:pubmed |
| pubmed-article:2719911 | pubmed:volume | 28 | lld:pubmed |
| pubmed-article:2719911 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2719911 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2719911 | pubmed:pagination | 1478-83 | lld:pubmed |
| pubmed-article:2719911 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2719911 | pubmed:meshHeading | pubmed-meshheading:2719911-... | lld:pubmed |
| pubmed-article:2719911 | pubmed:meshHeading | pubmed-meshheading:2719911-... | lld:pubmed |
| pubmed-article:2719911 | pubmed:meshHeading | pubmed-meshheading:2719911-... | lld:pubmed |
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| pubmed-article:2719911 | pubmed:meshHeading | pubmed-meshheading:2719911-... | lld:pubmed |
| pubmed-article:2719911 | pubmed:meshHeading | pubmed-meshheading:2719911-... | lld:pubmed |
| pubmed-article:2719911 | pubmed:meshHeading | pubmed-meshheading:2719911-... | lld:pubmed |
| pubmed-article:2719911 | pubmed:meshHeading | pubmed-meshheading:2719911-... | lld:pubmed |
| pubmed-article:2719911 | pubmed:meshHeading | pubmed-meshheading:2719911-... | lld:pubmed |
| pubmed-article:2719911 | pubmed:meshHeading | pubmed-meshheading:2719911-... | lld:pubmed |
| pubmed-article:2719911 | pubmed:meshHeading | pubmed-meshheading:2719911-... | lld:pubmed |
| pubmed-article:2719911 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2719911 | pubmed:articleTitle | Comparison of polymerase insertion and extension kinetics of a series of O2-alkyldeoxythymidine triphosphates and O4-methyldeoxythymidine triphosphate. | lld:pubmed |
| pubmed-article:2719911 | pubmed:affiliation | Donner Laboratory, Lawrence Berkeley Laboratory, University of California, Berkeley 94720. | lld:pubmed |
| pubmed-article:2719911 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2719911 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2719911 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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