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pubmed-article:2719664pubmed:abstractTextThe metabolism of 14C- and 36Cl-labelled 1,6-dichloro-1,6-dideoxyfructose (DCF) was studied in the isolated perfused rat liver system. Dechlorination of DCF occurred in the liver and erythrocytes and was GSH-dependent. The GSH conjugate formed was identified by 13C and 1H n.m.r. as the 6-chlorofructos-1-yl-SG conjugate. It is proposed that the GS- anion attacks the low steady-state concentration of the reactive keto form of DCF and that the conjugate formed cyclizes to the dominant beta-anomer. 6-Chlorofructos-1-yl-SG conjugate of hepatic origin is excreted into bile, whereas that produced in erythrocytes does not enter the liver.lld:pubmed
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pubmed-article:2719664pubmed:pagination537-43lld:pubmed
pubmed-article:2719664pubmed:dateRevised2009-11-18lld:pubmed
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pubmed-article:2719664pubmed:articleTitleGlutathione-dependent dechlorination of 1,6-dichloro-1,6-dideoxyfructose.lld:pubmed
pubmed-article:2719664pubmed:affiliationDepartment of Biochemistry, University of Wales College of Cardiff, U.K.lld:pubmed
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