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pubmed-article:2689228pubmed:abstractTextIntracellular Ca2+ levels were monitored in single, acutely isolated mouse islets of Langerhans by dual emission Indo-1 fluorometry. High-frequency (3.1 min-1) [Ca2+]i oscillations with a brief rising time (1-2 s) and 10 s half-width ('fast' oscillations) were detected in 11 mM glucose. Raising the glucose concentration to 16.7 mM increased the duration of these oscillations, which were otherwise absent in 5.5 mM glucose. [Ca2+]i waves of lower frequency (0.5 min-1) and longer rising time ('slow' oscillations) were also recorded. The data indicate that "fast" oscillations are directly related to beta-cell bursting electrical activity, and suggest the existence of extensive networks of electrically coupled cells in the islet.lld:pubmed
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pubmed-article:2689228pubmed:articleTitleGlucose-induced oscillations of intracellular Ca2+ concentration resembling bursting electrical activity in single mouse islets of Langerhans.lld:pubmed
pubmed-article:2689228pubmed:affiliationDepartment of Physiology, School of Medicine, University of Alicante, Spain.lld:pubmed
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