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pubmed-article:2680630pubmed:dateCreated1989-12-18lld:pubmed
pubmed-article:2680630pubmed:abstractTextThe force generated by the radiation pressure of a low power laser beam induces an optical trap which may be used to manipulate sperm. We studied the effect of the optical trap on sperm motility. A Nd:YAG laser beam was coupled to a conventional microscope and focused into the viewing plane by the objective lens. Sperm were caught in the trap and manipulated by a joy stick controlled motorized stage. After different exposure periods, the velocity and patterns were analysed by a computerized image processor. There were minor changes in sperm velocity when exposed to the trap for 30 seconds or less. A gradual decrease in the mean linear velocity was observed after 45 seconds of exposure. This optical micromanipulator may also be useful for studying the force generated by a single spermatozoa and evaluating the influence of drugs on motility.lld:pubmed
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pubmed-article:2680630pubmed:authorpubmed-author:BernsM WMWlld:pubmed
pubmed-article:2680630pubmed:authorpubmed-author:WrightW HWHlld:pubmed
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pubmed-article:2680630pubmed:pagination870-3lld:pubmed
pubmed-article:2680630pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:2680630pubmed:year1989lld:pubmed
pubmed-article:2680630pubmed:articleTitleMicromanipulation of sperm by a laser generated optical trap.lld:pubmed
pubmed-article:2680630pubmed:affiliationBeckman Laser Institute, University of California, Irvine.lld:pubmed
pubmed-article:2680630pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:2680630pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
pubmed-article:2680630pubmed:publicationTypeResearch Support, U.S. Gov't, Non-P.H.S.lld:pubmed
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