| pubmed-article:2678103 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2678103 | lifeskim:mentions | umls-concept:C0035668 | lld:lifeskim |
| pubmed-article:2678103 | lifeskim:mentions | umls-concept:C0021920 | lld:lifeskim |
| pubmed-article:2678103 | lifeskim:mentions | umls-concept:C1519249 | lld:lifeskim |
| pubmed-article:2678103 | lifeskim:mentions | umls-concept:C0678594 | lld:lifeskim |
| pubmed-article:2678103 | lifeskim:mentions | umls-concept:C0037791 | lld:lifeskim |
| pubmed-article:2678103 | lifeskim:mentions | umls-concept:C0441843 | lld:lifeskim |
| pubmed-article:2678103 | pubmed:issue | 19 | lld:pubmed |
| pubmed-article:2678103 | pubmed:dateCreated | 1989-11-9 | lld:pubmed |
| pubmed-article:2678103 | pubmed:abstractText | The group I self-splicing introns act at exon-intron junctions without recognizing a particular sequence. In order to understand splice-site selection, we have developed an assay system based on the Tetrahymena ribozyme to allow the study of numerous 5'-splice-site variants. Cleavage at the correct site requires formation of the correct secondary structure and occurs most efficiently within a 3-base-pair window centered on base pair 5 from the bottom of the P1 stem. Within this window the ribozyme recognizes and cleaves at a "wobble" base pair; the base pair above the cleavage site also influences splicing efficiency. The recognition of RNA structure rather than sequence explains the ability of these transposable introns to splice out of a variety of sequence contexts. | lld:pubmed |
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| pubmed-article:2678103 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2678103 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2678103 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2678103 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2678103 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:2678103 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:2678103 | pubmed:author | pubmed-author:SzostakJ WJW | lld:pubmed |
| pubmed-article:2678103 | pubmed:author | pubmed-author:CormackB PBP | lld:pubmed |
| pubmed-article:2678103 | pubmed:author | pubmed-author:DoudnaJ AJA | lld:pubmed |
| pubmed-article:2678103 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2678103 | pubmed:volume | 86 | lld:pubmed |
| pubmed-article:2678103 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2678103 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2678103 | pubmed:pagination | 7402-6 | lld:pubmed |
| pubmed-article:2678103 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:2678103 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2678103 | pubmed:articleTitle | RNA structure, not sequence, determines the 5' splice-site specificity of a group I intron. | lld:pubmed |
| pubmed-article:2678103 | pubmed:affiliation | Department of Molecular Biology, Massachusetts General Hospital, Boston 02114. | lld:pubmed |
| pubmed-article:2678103 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2678103 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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