| pubmed-article:2627937 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2627937 | lifeskim:mentions | umls-concept:C0237401 | lld:lifeskim |
| pubmed-article:2627937 | lifeskim:mentions | umls-concept:C0007635 | lld:lifeskim |
| pubmed-article:2627937 | lifeskim:mentions | umls-concept:C0040284 | lld:lifeskim |
| pubmed-article:2627937 | lifeskim:mentions | umls-concept:C0005575 | lld:lifeskim |
| pubmed-article:2627937 | lifeskim:mentions | umls-concept:C0204727 | lld:lifeskim |
| pubmed-article:2627937 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:2627937 | lifeskim:mentions | umls-concept:C1705425 | lld:lifeskim |
| pubmed-article:2627937 | lifeskim:mentions | umls-concept:C0181496 | lld:lifeskim |
| pubmed-article:2627937 | lifeskim:mentions | umls-concept:C0242392 | lld:lifeskim |
| pubmed-article:2627937 | lifeskim:mentions | umls-concept:C1522485 | lld:lifeskim |
| pubmed-article:2627937 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:2627937 | pubmed:dateCreated | 1990-4-26 | lld:pubmed |
| pubmed-article:2627937 | pubmed:abstractText | A method was developed to isolate cell surface proteins by a simple two-step procedure. Hepatocyte cell surface proteins were labeled by a cleavable biotin derivative in a covalent pulse reaction. Under the described conditions, NHS-SS-biotin proved to be an impermeant, cell surface-specific label which does not affect the impermeant, cell surface-specific label which does not affect the viability of rat hepatocytes. Biotinylated cell surface proteins could be selectively separated under non-denaturing conditions from non-biotinylated proteins and biotin-containing carboxylases by avidin affinity chromatography and sulfhydryl-mediated elution. Subsequent to alkylation of the eluted protein, individual cell surface proteins could be isolated by immunoprecipitation as shown for a selected Mr 120,000 glycoprotein gp120 of the hepatocyte plasma membrane. Using this technique, a transit time of gp120 from the endoplasmic reticulum to the cell surface of 2 h was determined. The results show that the combination of labeling with a cleavable biotin derivative, non-denaturing avidin affinity chromatography and immunoprecipitation is a useful method to isolate and study individual cell surface proteins. | lld:pubmed |
| pubmed-article:2627937 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2627937 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2627937 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2627937 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:2627937 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2627937 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:2627937 | pubmed:issn | 0171-9335 | lld:pubmed |
| pubmed-article:2627937 | pubmed:author | pubmed-author:ReutterWW | lld:pubmed |
| pubmed-article:2627937 | pubmed:author | pubmed-author:HodelJJ | lld:pubmed |
| pubmed-article:2627937 | pubmed:author | pubmed-author:TauberRR | lld:pubmed |
| pubmed-article:2627937 | pubmed:author | pubmed-author:BuschGG | lld:pubmed |
| pubmed-article:2627937 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2627937 | pubmed:volume | 50 | lld:pubmed |
| pubmed-article:2627937 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2627937 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2627937 | pubmed:pagination | 257-62 | lld:pubmed |
| pubmed-article:2627937 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:2627937 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2627937 | pubmed:articleTitle | Selective isolation of individual cell surface proteins from tissue culture cells by a cleavable biotin label. | lld:pubmed |
| pubmed-article:2627937 | pubmed:affiliation | Institut für Klinische Chemie und Biochemie, Freie Universität, Berlin-West/Federal Republic of Germany. | lld:pubmed |
| pubmed-article:2627937 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2627937 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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