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pubmed-article:2584170pubmed:abstractTextThe preservation of micro-organisms that may be found on the skin was studied by storage in liquid media at--70 degrees C. In the first part of the study the performance of 12 varieties of suspending media was evaluated with pure cultures of 17 species of micro-organisms maintained in the laboratory. After storage for 1 year the best medium (Oxoid Nutrient Broth with 15% glycerol) showed a mean survival for all organisms studied of 83.8%, with no significant differences between organisms. Even the worst medium (distilled water) permitted greater than 40% survival at 1 year. No changes in the characteristics of these micro-organisms were detected after 6 months storage in glycerol broth. In the second part of the study nose swabs were suspended in one representative medium (Bacto Nutrient Broth containing 7% glycerol). The mean percentage survival of staphylococci in these suspensions after 1 year's storage at - 70 degrees C was 75.4%. These results indicate that coagulase-negative coagulase-negative staphylococci in samples of skin flora may be stored under these conditions for long periods, greatly reducing the work-load in epidemiological studies of infection.lld:pubmed
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pubmed-article:2584170pubmed:authorpubmed-author:NobleW CWClld:pubmed
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pubmed-article:2584170pubmed:authorpubmed-author:LudlamH AHAlld:pubmed
pubmed-article:2584170pubmed:authorpubmed-author:NwachukwuBBlld:pubmed
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pubmed-article:2584170pubmed:volume67lld:pubmed
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pubmed-article:2584170pubmed:pagination417-23lld:pubmed
pubmed-article:2584170pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:2584170pubmed:year1989lld:pubmed
pubmed-article:2584170pubmed:articleTitleThe preservation of micro-organisms in biological specimens stored at-70 degrees C.lld:pubmed
pubmed-article:2584170pubmed:affiliationDivision of Microbiology, UMDS, St Thomas' Hospital, London, UK.lld:pubmed
pubmed-article:2584170pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:2584170pubmed:publicationTypeComparative Studylld:pubmed
pubmed-article:2584170pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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