| pubmed-article:2580832 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2580832 | lifeskim:mentions | umls-concept:C0596901 | lld:lifeskim |
| pubmed-article:2580832 | lifeskim:mentions | umls-concept:C0001473 | lld:lifeskim |
| pubmed-article:2580832 | lifeskim:mentions | umls-concept:C0441587 | lld:lifeskim |
| pubmed-article:2580832 | lifeskim:mentions | umls-concept:C0597484 | lld:lifeskim |
| pubmed-article:2580832 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:2580832 | pubmed:dateCreated | 1985-5-31 | lld:pubmed |
| pubmed-article:2580832 | pubmed:abstractText | Insertion of the alpha- and beta-subunits of amphibian epithelial Na+,K+-ATPase into pancreatic microsomes in cell-free systems was shown to be the same as into membranes of intact cells. The glycoproteic beta-subunit was observed to be cotranslationally inserted into endoplasmic reticulum membranes and to adopt a different pattern of N-linked core and terminal sugars in two different amphibian species. The beta-subunit lacks a cleavable signal sequence but quantitative membrane integration required membrane addition at the start of synthesis. Proteolysis of beta-subunit assembled in vitro indicated a cleavable cytoplasmic domain of about 2000 daltons. The catalytic 98-kilodalton alpha-subunit was also membrane-associated during its synthesis in an alkali-resistant fashion and independent of newly synthesized beta-subunit. In contrast to the beta-subunit, membrane integration of the alpha-subunit was possible as late as a time point in its synthesis which corresponded to about 1/3-1/2 of completion of the nascent chain. A small 34 kDa trypsin-resistant fragment of the alpha-subunit was produced at an early stage of synthesis both in the intact cell and in the cell-free system. These results suggest that membrane insertion of both alpha- and beta-subunit occurs during their synthesis but with a different time course. | lld:pubmed |
| pubmed-article:2580832 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2580832 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2580832 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2580832 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:2580832 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2580832 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:2580832 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:2580832 | pubmed:author | pubmed-author:GeeringKK | lld:pubmed |
| pubmed-article:2580832 | pubmed:author | pubmed-author:RossierB CBC | lld:pubmed |
| pubmed-article:2580832 | pubmed:author | pubmed-author:MeyerD IDI | lld:pubmed |
| pubmed-article:2580832 | pubmed:author | pubmed-author:KraehenbühlJ... | lld:pubmed |
| pubmed-article:2580832 | pubmed:author | pubmed-author:PaccolatM PMP | lld:pubmed |
| pubmed-article:2580832 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2580832 | pubmed:day | 25 | lld:pubmed |
| pubmed-article:2580832 | pubmed:volume | 260 | lld:pubmed |
| pubmed-article:2580832 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2580832 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2580832 | pubmed:pagination | 5154-60 | lld:pubmed |
| pubmed-article:2580832 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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| pubmed-article:2580832 | pubmed:meshHeading | pubmed-meshheading:2580832-... | lld:pubmed |
| pubmed-article:2580832 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:2580832 | pubmed:articleTitle | Membrane insertion of alpha- and beta-subunits of Na+,K+-ATPase. | lld:pubmed |
| pubmed-article:2580832 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2580832 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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