| pubmed-article:2550051 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2550051 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:2550051 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:2550051 | lifeskim:mentions | umls-concept:C0966207 | lld:lifeskim |
| pubmed-article:2550051 | lifeskim:mentions | umls-concept:C1705965 | lld:lifeskim |
| pubmed-article:2550051 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:2550051 | lifeskim:mentions | umls-concept:C0679058 | lld:lifeskim |
| pubmed-article:2550051 | lifeskim:mentions | umls-concept:C0087052 | lld:lifeskim |
| pubmed-article:2550051 | lifeskim:mentions | umls-concept:C1547699 | lld:lifeskim |
| pubmed-article:2550051 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:2550051 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
| pubmed-article:2550051 | pubmed:issue | 13 | lld:pubmed |
| pubmed-article:2550051 | pubmed:dateCreated | 1989-10-13 | lld:pubmed |
| pubmed-article:2550051 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2550051 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2550051 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2550051 | pubmed:abstractText | Tissue kallikreins are a group of serine proteases which may function as peptide hormone processing enzymes. Two rat kallikrein genomic clones (RSKG-5 and RSKG-50) were sequenced and characterized. The rat tonin gene and a kallikrein-like gene were found in clones RSKG-5 and RSKG-50, respectively. The tonin gene is 4146 base pairs in length, with both the variant CCAAA and TTTAAA boxes in the 5'-end region and an AATAAA polyadenylation signal at the 3' end of the gene. It has five exons which are separated by four introns. Sequence analysis of 3.7-kb 5' upstream and 7.5-kb 3' downstream of the tonin gene failed to reveal a second kallikrein gene. Sequence comparisons of the RSKG-5 exons with tonin cDNA revealed that only one base in the 3'-noncoding region was different from that in the previously reported rat tonin cDNA. Characteristic TC- and TG-repeated sequences were also found in the first and second introns of the tonin gene. The tonin gene encodes a preprotonin of 259 amino acids (aa). The active enzyme consists of 235 aa and is preceded by a deduced signal peptide of 17 aa and a profragment of 7 aa. Northern blot analysis indicates that RSKG-5 is expressed in a sex-dependent manner in rat submandibular gland, with a higher level expressed in males. The RSKG-50 gene was truncated at an EcoRI site in the second intron, excluding its 5' end. Compared to the coding sequence of pancreatic kallikrein, 12 nucleotides have been deleted in exon 3 of the RSKG-50 gene. The nucleotide sequences of the third, fourth, and fifth exons of the RSKG-50 gene encode a polypeptide of 188 aa residues. The translated peptide is 80% homologous to rat pancreatic kallikrein and 75% homologous to rat tonin in the corresponding regions. Key residues in the RSKG-50 gene product indicate a serine protease with kallikrein-like cleavage specificity at basic amino acids. | lld:pubmed |
| pubmed-article:2550051 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2550051 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2550051 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2550051 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2550051 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2550051 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2550051 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2550051 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2550051 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2550051 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:2550051 | pubmed:issn | 0006-2960 | lld:pubmed |
| pubmed-article:2550051 | pubmed:author | pubmed-author:ChaoJJ | lld:pubmed |
| pubmed-article:2550051 | pubmed:author | pubmed-author:ChanMM | lld:pubmed |
| pubmed-article:2550051 | pubmed:author | pubmed-author:ShaiS YSY | lld:pubmed |
| pubmed-article:2550051 | pubmed:author | pubmed-author:Woodley-Mille... | lld:pubmed |
| pubmed-article:2550051 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2550051 | pubmed:day | 27 | lld:pubmed |
| pubmed-article:2550051 | pubmed:volume | 28 | lld:pubmed |
| pubmed-article:2550051 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2550051 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2550051 | pubmed:pagination | 5334-43 | lld:pubmed |
| pubmed-article:2550051 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2550051 | pubmed:meshHeading | pubmed-meshheading:2550051-... | lld:pubmed |
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| pubmed-article:2550051 | pubmed:meshHeading | pubmed-meshheading:2550051-... | lld:pubmed |
| pubmed-article:2550051 | pubmed:meshHeading | pubmed-meshheading:2550051-... | lld:pubmed |
| pubmed-article:2550051 | pubmed:meshHeading | pubmed-meshheading:2550051-... | lld:pubmed |
| pubmed-article:2550051 | pubmed:meshHeading | pubmed-meshheading:2550051-... | lld:pubmed |
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| pubmed-article:2550051 | pubmed:meshHeading | pubmed-meshheading:2550051-... | lld:pubmed |
| pubmed-article:2550051 | pubmed:meshHeading | pubmed-meshheading:2550051-... | lld:pubmed |
| pubmed-article:2550051 | pubmed:meshHeading | pubmed-meshheading:2550051-... | lld:pubmed |
| pubmed-article:2550051 | pubmed:meshHeading | pubmed-meshheading:2550051-... | lld:pubmed |
| pubmed-article:2550051 | pubmed:meshHeading | pubmed-meshheading:2550051-... | lld:pubmed |
| pubmed-article:2550051 | pubmed:meshHeading | pubmed-meshheading:2550051-... | lld:pubmed |
| pubmed-article:2550051 | pubmed:meshHeading | pubmed-meshheading:2550051-... | lld:pubmed |
| pubmed-article:2550051 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2550051 | pubmed:articleTitle | Characterization of genes encoding rat tonin and a kallikrein-like serine protease. | lld:pubmed |
| pubmed-article:2550051 | pubmed:affiliation | Department of Biochemistry, Medical University of South Carolina, Charleston 29425. | lld:pubmed |
| pubmed-article:2550051 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2550051 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:2550051 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| entrez-gene:24841 | entrezgene:pubmed | pubmed-article:2550051 | lld:entrezgene |
| entrez-gene:292872 | entrezgene:pubmed | pubmed-article:2550051 | lld:entrezgene |
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