| pubmed-article:2544867 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2544867 | lifeskim:mentions | umls-concept:C0015161 | lld:lifeskim |
| pubmed-article:2544867 | lifeskim:mentions | umls-concept:C0012920 | lld:lifeskim |
| pubmed-article:2544867 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:2544867 | lifeskim:mentions | umls-concept:C0521115 | lld:lifeskim |
| pubmed-article:2544867 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:2544867 | pubmed:dateCreated | 1989-8-3 | lld:pubmed |
| pubmed-article:2544867 | pubmed:abstractText | We have developed a procedure for the simultaneous purification of DNA topoisomerase I and II from calf thymus. Both enzymes were first extracted from isolated nucleoprotein complexes. After batchwise chromatography on hydroxylapatite the two enzyme activities were separated on a FPLC phenylsuperose column. The enzymes were further purified by a second chromatography on phenylsepharose (topo I) or FPLC Mono Q (topo II). The purification can be finished within three days, yielding 0.5-1.0 mg quantities of homogeneous, enzymatic active preparations of the two proteins from 200 g of starting material. | lld:pubmed |
| pubmed-article:2544867 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2544867 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2544867 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2544867 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2544867 | pubmed:issn | 0032-7484 | lld:pubmed |
| pubmed-article:2544867 | pubmed:author | pubmed-author:RichterAA | lld:pubmed |
| pubmed-article:2544867 | pubmed:author | pubmed-author:StrausfeldUU | lld:pubmed |
| pubmed-article:2544867 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2544867 | pubmed:volume | 19 | lld:pubmed |
| pubmed-article:2544867 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2544867 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2544867 | pubmed:pagination | 37-48 | lld:pubmed |
| pubmed-article:2544867 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:2544867 | pubmed:meshHeading | pubmed-meshheading:2544867-... | lld:pubmed |
| pubmed-article:2544867 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2544867 | pubmed:articleTitle | Simultaneous purification of DNA topoisomerase I and II from eukaryotic cells. | lld:pubmed |
| pubmed-article:2544867 | pubmed:affiliation | Universität Konstanz, Fakultät für Biologie, FRG. | lld:pubmed |
| pubmed-article:2544867 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2544867 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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