| pubmed-article:2541221 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2541221 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2541221 | lifeskim:mentions | umls-concept:C0439849 | lld:lifeskim |
| pubmed-article:2541221 | lifeskim:mentions | umls-concept:C0014792 | lld:lifeskim |
| pubmed-article:2541221 | lifeskim:mentions | umls-concept:C0079731 | lld:lifeskim |
| pubmed-article:2541221 | lifeskim:mentions | umls-concept:C1327616 | lld:lifeskim |
| pubmed-article:2541221 | lifeskim:mentions | umls-concept:C1171362 | lld:lifeskim |
| pubmed-article:2541221 | lifeskim:mentions | umls-concept:C0004358 | lld:lifeskim |
| pubmed-article:2541221 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:2541221 | lifeskim:mentions | umls-concept:C0598666 | lld:lifeskim |
| pubmed-article:2541221 | lifeskim:mentions | umls-concept:C1515670 | lld:lifeskim |
| pubmed-article:2541221 | lifeskim:mentions | umls-concept:C1521733 | lld:lifeskim |
| pubmed-article:2541221 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:2541221 | pubmed:dateCreated | 1989-6-9 | lld:pubmed |
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| pubmed-article:2541221 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2541221 | pubmed:abstractText | To study the biology of cold agglutinin disease we previously established EBV-transformed B cell clones isolated from a patient with splenic lymphoma of an early plasmacytic cell type and immune hemolysis due to an anti-Pr2 cold agglutinin. These clones had an aberrant chromosomal marker identical to the patient's B cell lymphoma and each secreted IgMk anti-Pr2 similar to the pathologic autoantibody in the serum of the patient. In this study, we have further investigated the Pr2-specific autoimmune response through nucleotide sequencing of VH and VL region genes. We have shown that the seven clones share the same VDJ/VJ gene segments and junctional elements confirming their clonal origin. The VH sequences were 88% homologous to a VHI germline gene while the VL sequences were 97% homologous to a VkIII germline gene. Only 4 somatic mutations (3 silent and 1 conservative) were found in greater than 5,000 bp sequenced, suggesting that a low mutation rate existed. Based on a tumor mass of 10(12) cells and a minimum of 40 divisions, we estimated the somatic mutation rate to be 4.45 x 10(-5) m/bp/d. This somatic mutation rate is similar to those estimated for acute lymphocytic leukemia (pre-B cell) and chronic lymphocytic leukemia (intermediate B cell), but significantly lower than the mutation frequency in follicular lymphomas (activated B cell). We propose that the difference in somatic mutation frequency of a B cell tumor may be related to the stage of B cell differentiation. In addition, the low mutation frequency observed in the Pr2-specific B cell tumor may also reflect, in part, selection by autoantigen to conserve sIg structure and specificity. | lld:pubmed |
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| pubmed-article:2541221 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2541221 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2541221 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2541221 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2541221 | pubmed:month | May | lld:pubmed |
| pubmed-article:2541221 | pubmed:issn | 0022-1007 | lld:pubmed |
| pubmed-article:2541221 | pubmed:author | pubmed-author:LitwinSS | lld:pubmed |
| pubmed-article:2541221 | pubmed:author | pubmed-author:CarmackC ECE | lld:pubmed |
| pubmed-article:2541221 | pubmed:author | pubmed-author:SilbersteinL... | lld:pubmed |
| pubmed-article:2541221 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2541221 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:2541221 | pubmed:volume | 169 | lld:pubmed |
| pubmed-article:2541221 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2541221 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2541221 | pubmed:pagination | 1631-43 | lld:pubmed |
| pubmed-article:2541221 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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