| pubmed-article:2536046 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2536046 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2536046 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:2536046 | lifeskim:mentions | umls-concept:C0027950 | lld:lifeskim |
| pubmed-article:2536046 | lifeskim:mentions | umls-concept:C0178719 | lld:lifeskim |
| pubmed-article:2536046 | lifeskim:mentions | umls-concept:C0023810 | lld:lifeskim |
| pubmed-article:2536046 | lifeskim:mentions | umls-concept:C0085416 | lld:lifeskim |
| pubmed-article:2536046 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:2536046 | lifeskim:mentions | umls-concept:C2349975 | lld:lifeskim |
| pubmed-article:2536046 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:2536046 | pubmed:dateCreated | 1989-2-23 | lld:pubmed |
| pubmed-article:2536046 | pubmed:abstractText | Lipopolysaccharide (LPS) pretreatment "primes" neutrophils to release increased amounts of superoxide anion (O2-) when stimulated. We investigated the molecular basis of this enhanced activity. Comparison of kinetic parameters of the respiratory burst NADPH oxidase in unstimulated LPS-primed and control neutrophils disclosed a similar Km for NADPH and no difference was seen in the content of cytochrome b. Pertussis toxin, which inhibits some G proteins, did not prevent priming. Change in membrane potential (delta psi) was five-fold greater in LPS-primed cells and paralleled the increased O2- release. Cytofluorographic analysis indicated that the increased change in delta psi was due to the creation of a new population of active cells. Changes in the concentration of intracellular free Ca2+ ([Ca2+]i) are believed to antecede changes in delta psi. There was a consistent increment (67 +/- 8%, n = 12) in resting [Ca2+]i in cells preincubated with LPS compared with control. When stimulated, the peak [Ca2+]i was significantly higher in LPS-primed cells. Ca2+-dependent protein kinase C activity was unaltered in resting and FMLP-stimulated neutrophils preexposed to LPS. Addition to cells of the intracellular Ca2+ chelator MAPTAM before preincubation with LPS blocked the changes in [Ca2+]i and the enhanced respiratory burst that characterize LPS priming. The increased resting [Ca2+]i in LPS-primed cells may enhance stimulus-induced cellular activity by modifying a Ca2+-dependent step in signal transduction. | lld:pubmed |
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| pubmed-article:2536046 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2536046 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2536046 | pubmed:citationSubset | AIM | lld:pubmed |
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| pubmed-article:2536046 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2536046 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:2536046 | pubmed:issn | 0021-9738 | lld:pubmed |
| pubmed-article:2536046 | pubmed:author | pubmed-author:JohnstonR... | lld:pubmed |
| pubmed-article:2536046 | pubmed:author | pubmed-author:PabstM JMJ | lld:pubmed |
| pubmed-article:2536046 | pubmed:author | pubmed-author:PhillipsW AWA | lld:pubmed |
| pubmed-article:2536046 | pubmed:author | pubmed-author:ForehandJ RJR | lld:pubmed |
| pubmed-article:2536046 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2536046 | pubmed:volume | 83 | lld:pubmed |
| pubmed-article:2536046 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2536046 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2536046 | pubmed:pagination | 74-83 | lld:pubmed |
| pubmed-article:2536046 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:2536046 | pubmed:meshHeading | pubmed-meshheading:2536046-... | lld:pubmed |
| pubmed-article:2536046 | pubmed:meshHeading | pubmed-meshheading:2536046-... | lld:pubmed |
