| pubmed-article:2521995 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2521995 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:2521995 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:2521995 | lifeskim:mentions | umls-concept:C0004799 | lld:lifeskim |
| pubmed-article:2521995 | lifeskim:mentions | umls-concept:C0062503 | lld:lifeskim |
| pubmed-article:2521995 | lifeskim:mentions | umls-concept:C0022663 | lld:lifeskim |
| pubmed-article:2521995 | lifeskim:mentions | umls-concept:C0243127 | lld:lifeskim |
| pubmed-article:2521995 | lifeskim:mentions | umls-concept:C1515655 | lld:lifeskim |
| pubmed-article:2521995 | lifeskim:mentions | umls-concept:C0443331 | lld:lifeskim |
| pubmed-article:2521995 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:2521995 | pubmed:dateCreated | 1989-3-28 | lld:pubmed |
| pubmed-article:2521995 | pubmed:abstractText | The metabolic turnover of rat glomerular proteoglycans in vivo was investigated. Newly synthesized proteoglycans were labeled during a 7-h period after injecting sodium [35S]sulfate intraperitoneally. At the end of the labeling period a chase dose of sodium sulfate was given. Subsequently at defined times (0-163 h) the kidneys were perfused in situ with 0.01% cetylpyridinium chloride in phosphate-buffered saline to maximize the recovery of 35S-proteoglycans. Glomeruli were isolated from the renal cortex and analyzed for 35S-proteoglycans by autoradiographic, biochemical, and immunochemical methods. Grain counting of autoradiographs revealed a complex turnover pattern of 35S-labeled macromolecules, commencing with a rapid phase followed by a slower phase. Biochemical analysis confirmed the biphasic pattern and showed that the total population of [35S]heparan sulfate proteoglycans had a metabolic half-life (t1/2) of 20 and 60 h in the early and late phases, respectively. Heparan sulfate proteoglycans accounted for 80% of total 35S-proteoglycans, the remainder being chondroitin/dermatan sulfate proteoglycans. Whole glomeruli were extracted with 4% 3-[(cholamidopropyl)dimethy-lammonio]-1-propanesulfonate-4 M guanidine hydrochloride, a procedure which solubilized greater than 95% of the 35S-labeled macromolecules. Of these 11-13% was immunoprecipitated by an antiserum against heparan sulfate proteoglycan which, in immunolocalization experiments, showed specificity for staining the basement membrane of rat glomeruli. Autoradiographic analysis showed that 18% of total radioactivity present at the end of the labeling period was associated with the glomerular basement membrane. The glomerular basement membrane [35S]heparan sulfate proteoglycans, identified by immunoprecipitation, have a very rapid turnover with an initial phase, t1/2 = 5 h, and a later phase t1/2 = 20 h. | lld:pubmed |
| pubmed-article:2521995 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2521995 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2521995 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2521995 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2521995 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2521995 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2521995 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2521995 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2521995 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2521995 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2521995 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2521995 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2521995 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:2521995 | pubmed:issn | 0003-9861 | lld:pubmed |
| pubmed-article:2521995 | pubmed:author | pubmed-author:WilliamsM AMA | lld:pubmed |
| pubmed-article:2521995 | pubmed:author | pubmed-author:MasonR MRM | lld:pubmed |
| pubmed-article:2521995 | pubmed:author | pubmed-author:DaviesMM | lld:pubmed |
| pubmed-article:2521995 | pubmed:author | pubmed-author:CouchmanJ RJR | lld:pubmed |
| pubmed-article:2521995 | pubmed:author | pubmed-author:BeavanL ALA | lld:pubmed |
| pubmed-article:2521995 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2521995 | pubmed:volume | 269 | lld:pubmed |
| pubmed-article:2521995 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2521995 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2521995 | pubmed:pagination | 576-85 | lld:pubmed |
| pubmed-article:2521995 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:2521995 | pubmed:meshHeading | pubmed-meshheading:2521995-... | lld:pubmed |
| pubmed-article:2521995 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2521995 | pubmed:articleTitle | In vivo turnover of the basement membrane and other heparan sulfate proteoglycans of rat glomerulus. | lld:pubmed |
| pubmed-article:2521995 | pubmed:affiliation | Department of Biochemistry, Charing Cross and Westminster Medical School, London, United Kingdom. | lld:pubmed |
| pubmed-article:2521995 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2521995 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2521995 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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