| pubmed-article:2517395 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2517395 | lifeskim:mentions | umls-concept:C2246376 | lld:lifeskim |
| pubmed-article:2517395 | lifeskim:mentions | umls-concept:C1545588 | lld:lifeskim |
| pubmed-article:2517395 | lifeskim:mentions | umls-concept:C0205369 | lld:lifeskim |
| pubmed-article:2517395 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:2517395 | lifeskim:mentions | umls-concept:C1518434 | lld:lifeskim |
| pubmed-article:2517395 | lifeskim:mentions | umls-concept:C0066110 | lld:lifeskim |
| pubmed-article:2517395 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:2517395 | pubmed:dateCreated | 1990-5-31 | lld:pubmed |
| pubmed-article:2517395 | pubmed:abstractText | Endonuclease protection or "footprinting" analysis is a powerful technique for identifying the nucleotides involved in a protein-DNA interaction. DNase I is the most often employed endonucleolytic agent; however, this endonuclease does not exhibit the true nonsequence-specific cleavage desired for this type of analysis. Methidiumpropyl-EDTA (MPE) is a synthetic DNA intercalator that cleaves DNA in the presence of ferrous ion and oxygen. Cleavage by MPE exhibits no sequence specificity, a characteristic that makes this reagent better suited for protection analysis. Here we report a generally applicable technique for MPE protection (or "footprinting") analysis of specific DNA-protein complexes from a crude nuclear extract. We have used this method to identify the nucleotides of the immunoglobulin (Ig) heavy chain promoter region that are involved in complex formation with a protein that binds the octameric sequence ATGCAAAT, and we compare our results to those obtained previously using DNase I. | lld:pubmed |
| pubmed-article:2517395 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2517395 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2517395 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2517395 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2517395 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2517395 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2517395 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2517395 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2517395 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2517395 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2517395 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2517395 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2517395 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2517395 | pubmed:month | May | lld:pubmed |
| pubmed-article:2517395 | pubmed:issn | 0736-6205 | lld:pubmed |
| pubmed-article:2517395 | pubmed:author | pubmed-author:TuckerP WPW | lld:pubmed |
| pubmed-article:2517395 | pubmed:author | pubmed-author:CapraJ DJD | lld:pubmed |
| pubmed-article:2517395 | pubmed:author | pubmed-author:TanY AYA | lld:pubmed |
| pubmed-article:2517395 | pubmed:author | pubmed-author:LandolfiN FNF | lld:pubmed |
| pubmed-article:2517395 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2517395 | pubmed:volume | 7 | lld:pubmed |
| pubmed-article:2517395 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2517395 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2517395 | pubmed:pagination | 500-4 | lld:pubmed |
| pubmed-article:2517395 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2517395 | pubmed:meshHeading | pubmed-meshheading:2517395-... | lld:pubmed |
| pubmed-article:2517395 | pubmed:meshHeading | pubmed-meshheading:2517395-... | lld:pubmed |
| pubmed-article:2517395 | pubmed:meshHeading | pubmed-meshheading:2517395-... | lld:pubmed |
| pubmed-article:2517395 | pubmed:meshHeading | pubmed-meshheading:2517395-... | lld:pubmed |
| pubmed-article:2517395 | pubmed:meshHeading | pubmed-meshheading:2517395-... | lld:pubmed |
| pubmed-article:2517395 | pubmed:meshHeading | pubmed-meshheading:2517395-... | lld:pubmed |
| pubmed-article:2517395 | pubmed:meshHeading | pubmed-meshheading:2517395-... | lld:pubmed |
| pubmed-article:2517395 | pubmed:meshHeading | pubmed-meshheading:2517395-... | lld:pubmed |
| pubmed-article:2517395 | pubmed:meshHeading | pubmed-meshheading:2517395-... | lld:pubmed |
| pubmed-article:2517395 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2517395 | pubmed:articleTitle | Protection analysis (or "footprinting") of specific protein-DNA complexes in crude nuclear extracts using methidiumpropyl-EDTA-iron (II). | lld:pubmed |
| pubmed-article:2517395 | pubmed:affiliation | University of Texas, Southwestern Medical Center. | lld:pubmed |
| pubmed-article:2517395 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2517395 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:2517395 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2517395 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2517395 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2517395 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2517395 | lld:pubmed |
