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pubmed-article:2508273pubmed:abstractTextBotulinum C2 toxin ADP-ribosylates actin in intact chicken embryo cells in a concentration-dependent manner. This effect correlates with an enhancement in the inhibitory potency of the respective cell lysates on DNAse I activity, indicating an increase in the cellular G-actin content of toxin-treated cells. The data support our view, that ADP-ribosylation of cellular actin with subsequent depolymerization of cytoskeleton-associated F-actin to monomeric G-actin is involved in the cytotoxic effects of botulinum C2 toxin. A model of the cytopathic action of actin-ADP-ribosylating toxins is presented.lld:pubmed
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pubmed-article:2508273pubmed:dateRevised2010-11-18lld:pubmed
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pubmed-article:2508273pubmed:year1989lld:pubmed
pubmed-article:2508273pubmed:articleTitleBotulinum C2 toxin treatment increases the G-actin pool in intact chicken cells: a model for the cytopathic action of actin-ADP-ribosylating toxins.lld:pubmed
pubmed-article:2508273pubmed:affiliationRudolf-Buchheim-Institut für Pharmakologie, Universität Giessen, F.R.G.lld:pubmed
pubmed-article:2508273pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:2508273pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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