| pubmed-article:2504085 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2504085 | lifeskim:mentions | umls-concept:C1123019 | lld:lifeskim |
| pubmed-article:2504085 | lifeskim:mentions | umls-concept:C0030524 | lld:lifeskim |
| pubmed-article:2504085 | lifeskim:mentions | umls-concept:C0014441 | lld:lifeskim |
| pubmed-article:2504085 | lifeskim:mentions | umls-concept:C0600324 | lld:lifeskim |
| pubmed-article:2504085 | lifeskim:mentions | umls-concept:C0065041 | lld:lifeskim |
| pubmed-article:2504085 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:2504085 | pubmed:dateCreated | 1989-9-15 | lld:pubmed |
| pubmed-article:2504085 | pubmed:abstractText | The use of lipoarabinomannan (LAM; obtained from Mycobacterium paratuberculosis) in and ELISA (LAM-ELISA) to test 75 sheep sera from a paratuberculosis-infected flock resulted in an approximate threefold increase in sensitivity (from 23.5% to 70.6%), compared with the use of Annau's polysaccharide in a complement fixation test (P-CFT). Even after manipulation of the LAM-ELISA cut-off value to produce a specificity of 100% to match that of the P-CFT, the sensitivity still was approximately twofold greater than that of the P-CFT. Anti-bovine monoclonal antiglobulin-enzyme conjugates matched commercially available anti-ovine polyclonal antiglobulin-enzyme conjugates with respect to sensitivity and specificity. False-positive results were found to be less frequent after combining 2 serodiagnostic tests, LAM-ELISA and D antigenagar gel immunodiffusion, resulting in an increase in specificity from 88.1% to 95.2%. The repeatability of true seropositive and seronegative results was found to be 89.5% and 91.1%, respectively, for sera obtained less than or equal to 1 month prior to slaughter and 91.7% and 95.5%, respectively, for reanalysis of sera obtained at the time of slaughter. | lld:pubmed |
| pubmed-article:2504085 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2504085 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2504085 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2504085 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2504085 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2504085 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2504085 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2504085 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2504085 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:2504085 | pubmed:issn | 0002-9645 | lld:pubmed |
| pubmed-article:2504085 | pubmed:author | pubmed-author:StewartR BRB | lld:pubmed |
| pubmed-article:2504085 | pubmed:author | pubmed-author:DuncanJ RJR | lld:pubmed |
| pubmed-article:2504085 | pubmed:author | pubmed-author:NielsenK HKH | lld:pubmed |
| pubmed-article:2504085 | pubmed:author | pubmed-author:SamaghB SBS | lld:pubmed |
| pubmed-article:2504085 | pubmed:author | pubmed-author:CornerA HAH | lld:pubmed |
| pubmed-article:2504085 | pubmed:author | pubmed-author:SugdenE AEA | lld:pubmed |
| pubmed-article:2504085 | pubmed:author | pubmed-author:TurcotteCC | lld:pubmed |
| pubmed-article:2504085 | pubmed:author | pubmed-author:BrooksB WBW | lld:pubmed |
| pubmed-article:2504085 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2504085 | pubmed:volume | 50 | lld:pubmed |
| pubmed-article:2504085 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2504085 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2504085 | pubmed:pagination | 850-4 | lld:pubmed |
| pubmed-article:2504085 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2504085 | pubmed:meshHeading | pubmed-meshheading:2504085-... | lld:pubmed |
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| pubmed-article:2504085 | pubmed:meshHeading | pubmed-meshheading:2504085-... | lld:pubmed |
| pubmed-article:2504085 | pubmed:meshHeading | pubmed-meshheading:2504085-... | lld:pubmed |
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| pubmed-article:2504085 | pubmed:meshHeading | pubmed-meshheading:2504085-... | lld:pubmed |
| pubmed-article:2504085 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2504085 | pubmed:articleTitle | Serodiagnosis of ovine paratuberculosis, using lipoarabinomannan in an enzyme-linked immunosorbent assay. | lld:pubmed |
| pubmed-article:2504085 | pubmed:affiliation | Agriculture Canada, Animal Disease Research Institute, Nepean, Ontario, Canada. | lld:pubmed |
| pubmed-article:2504085 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2504085 | pubmed:publicationType | Comparative Study | lld:pubmed |
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